Andersen O K, Volden G, Osterud B, Giercksky K E
Scand J Clin Lab Invest. 1986 Apr;46(2):143-9. doi: 10.3109/00365518609083650.
Tube migration of a fixed number of PMN cells in plasma (5 X 10(9) cells/l) was approximately reduced by 50% when 1 ml of cell suspension was exposed to 20 micrograms E. coli endotoxin in 100 microliter NaCl for 2 hours. Procoagulant activity in monocytes increased approximately eight-fold when 1 ml of whole blood was exposed to 2 ng endotoxin in 10 microliters NaCl for 2 hours. Chemiluminescence in both PMN cells (5 X 10(9) cells/l) and mononuclear cells (2 X 10(9) cells/l) in plasma was markedly increased when 100 microliters cell suspension was added to 100 microliters Luminol, exposed to 20 micrograms endotoxin in 100 microliters NaCl and tested immediately. Decreased lysosomal enzyme levels in PMN and mononuclear cells were demonstrated when 1 ml cells in plasma (the same cell numbers as aforementioned) were incubated for 4 hours at 37 degrees C with 200 micrograms endotoxin in 100 microliters NaCl. Similar results were obtained in human and porcine leucocytes, making the pig a suitable animal for studies of humoral and cellular reactions to infections complications following trauma and major surgery.
当1毫升细胞悬液在100微升氯化钠中与20微克大肠杆菌内毒素接触2小时时，血浆中固定数量的中性粒细胞（5×10⁹个细胞/升）的趋化移动约减少50%。当1毫升全血在10微升氯化钠中与2纳克内毒素接触2小时时，单核细胞中的促凝活性增加约8倍。当将100微升细胞悬液加入100微升鲁米诺中，在100微升氯化钠中与20微克内毒素接触并立即检测时，血浆中中性粒细胞（5×10⁹个细胞/升）和单核细胞（2×10⁹个细胞/升）的化学发光均显著增加。当血浆中的1毫升细胞（细胞数量与上述相同）在37℃下与100微升氯化钠中的200微克内毒素孵育4小时时，中性粒细胞和单核细胞中的溶酶体酶水平降低。在人和猪的白细胞中获得了类似结果，这使得猪成为研究创伤和大手术后感染并发症的体液和细胞反应的合适动物。
