Semeraro N, Colucci M, Mussoni L, Donati M B
Br J Exp Pathol. 1981 Dec;62(6):638-42.
We have investigated the ability of rat and rabbit leucocytes to generate procoagulant activity (PCA) in response to endotoxin in vitro and in vivo. On prolonged incubation with endotoxin (10 micrograms/ml f.c.) isolated rabbit leucocytes developed strong PCA as measured by clotting and amidolytic assay. In contrast, rat leucocytes failed to produce any PCA even in the presence of huge amounts of endotoxin (200 micrograms/mol f.c.). When rabbits were given two spaced endotoxin injections (25 micrograms/kg consistently showed marked PCA. Again, unlike in the rabbit, rat leucocytes obtained after 2 endotoxin injections (up to 2 mg/kg body wt) showed absolutely no PCA. These findings support the view that leucocytes are involved in endotoxin-induced disseminated intravascular coagulation (DIC) in rabbits. On the other hand the poor response of rat leucocytes to endotoxin might help explain the resistance of rats to DIC and Sanarelli-Shwartzman reaction.
我们研究了大鼠和兔白细胞在体外和体内对内毒素产生促凝血活性（PCA）的能力。将分离的兔白细胞与内毒素（10微克/毫升，腹腔注射）长时间孵育后，通过凝血和酰胺水解测定法测得其产生了很强的PCA。相比之下，即使存在大量内毒素（200微克/毫升，腹腔注射），大鼠白细胞也未能产生任何PCA。当给兔子间隔注射两次内毒素（25微克/千克）时，始终显示出明显的PCA。同样，与兔子不同，两次注射内毒素（高达2毫克/千克体重）后获得的大鼠白细胞完全没有显示出PCA。这些发现支持了白细胞参与兔子内毒素诱导的弥散性血管内凝血（DIC）的观点。另一方面，大鼠白细胞对内毒素的反应较差可能有助于解释大鼠对DIC和施瓦茨曼反应的抵抗力。
