McGee M P, Rothberger H, Lee T K
Thromb Haemost. 1984 Apr 30;51(2):228-31.
Rabbit mononuclear leukocytes isolated from a variety of anatomic sites were examined for ability to generate procoagulant activity in vitro. Marrow, blood and spleen mononuclear cell populations were found to differ functionally from lymph node, thymus and alveolar populations by having much greater ability to increase in tissue factor activity in response to an endotoxin stimulus. Thus, after incubation in the presence of endotoxin, leukocytes obtained from marrow, blood, and spleen were found to increase in procoagulant activity characterized as tissue factor by 832, 1942 and 12.6 fold, respectively. In contrast, pulmonary alveolar macrophages increased in tissue factor activity only by 2.8 fold, and lymph node and thymus mononuclear cells showed little or no increases. These functional differences, demonstrated by exposing the six cell populations to endotoxin under controlled conditions in vitro, likely explain the similar pattern of anatomic selectivity of leukocyte tissue factor increases reported to occur in vivo during endotoxemia and Shwartzman reactions (1).
对从各种解剖部位分离出的兔单核白细胞进行了体外产生促凝活性能力的检测。发现骨髓、血液和脾脏单核细胞群体在功能上与淋巴结、胸腺和肺泡群体不同，它们对内毒素刺激产生组织因子活性增加的能力要强得多。因此，在内毒素存在下孵育后，发现从骨髓、血液和脾脏获得的白细胞促凝活性分别增加了832倍、1942倍和12.6倍，其促凝活性表现为组织因子。相比之下，肺泡巨噬细胞的组织因子活性仅增加了2.8倍，而淋巴结和胸腺单核细胞几乎没有增加或没有增加。通过在体外受控条件下将六个细胞群体暴露于内毒素所证明的这些功能差异，可能解释了据报道在内毒素血症和施瓦茨曼反应期间体内发生的白细胞组织因子增加的类似解剖学选择性模式(1)。
