Pruitt Tyrell, Carter Caroline, Wang Xinlong, Wu Anqi, Liu Hanli
Department of Bioengineering, University of Texas at Arlington, 500 UTA Blvd, Arlington, TX 76019, USA.
UT Southwestern Medical Center, Department of Radiology, 5323 Harry Hines Blvd, Dallas, TX 75390, USA.
Metabolites. 2022 Jan 23;12(2):103. doi: 10.3390/metabo12020103.
Our group previously examined 8 min photobiomodulation (PBM) by 1064 nm laser on the human forearm in vivo to determine its significant effects on vascular hemodynamics and cytochrome c oxidase redox activity. Since PBM uses a wide array of wavelengths, in this paper, we investigated (i) whether different wavelengths of lasers induced different PBM effects, and (ii) if a light-emitting diode (LED) at a similar wavelength to a laser could induce similar PBM effects. A broadband near-infrared spectroscopy (bbNIRS) system was utilized to assess concentration changes in oxygenated hemoglobin (Δ[HbO]) and oxidized cytochrome c oxidase (Δ[oxCCO]) during and after PBM with lasers at 800 nm, 850 nm, and 1064 nm, as well as a LED at 810 nm. Two groups of 10 healthy participants were measured before, during, and after active and sham PBM on their forearms. All results were tested for significance using repeated measures ANOVA. Our results showed that (i) lasers at all three wavelengths enabled significant increases in Δ[HbO] and Δ[oxCCO] of the human forearm while the 1064 nm laser sustained the increases longer, and that (ii) the 810-nm LED with a moderate irradiance (≈135 mW/cm) induced measurable and significant rises in Δ[HbO] and Δ[oxCCO] with respect to the sham stimulation on the human forearm.
我们团队之前在人体前臂上进行了8分钟的1064纳米激光光生物调节（PBM）实验，以确定其对血管血流动力学和细胞色素c氧化酶氧化还原活性的显著影响。由于PBM使用多种波长，在本文中，我们研究了（i）不同波长的激光是否会诱导不同的PBM效应，以及（ii）与激光波长相似的发光二极管（LED）是否能诱导相似的PBM效应。利用宽带近红外光谱（bbNIRS）系统评估在使用800纳米、850纳米和1064纳米激光以及810纳米LED进行PBM期间和之后，氧合血红蛋白（Δ[HbO]）和氧化细胞色素c氧化酶（Δ[oxCCO]）的浓度变化。两组各10名健康参与者在其前臂进行主动和假PBM之前、期间和之后进行测量。所有结果均使用重复测量方差分析进行显著性检验。我们的结果表明，（i）所有三种波长的激光均能使人体前臂的Δ[HbO]和Δ[oxCCO]显著增加，而1064纳米激光使这种增加持续的时间更长，并且（ii）具有中等辐照度（≈135 mW/cm）的810纳米LED相对于人体前臂的假刺激，能诱导Δ[HbO]和Δ[oxCCO]出现可测量的显著升高。
