Argibay Bárbara, Campos Francisco, Perez-Mato María, Vieites-Prado Alba, Correa-Paz Clara, López-Arias Esteban, Da Silva-Candal Andrés, Moreno Vicente, Montero Carlos, Sobrino Tomás, Castillo José, Iglesias-Rey Ramón
Clinical Neurosciences Research Laboratory, Health Research Institute of Santiago de Compostela (IDIS), Clinical University Hospital, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.
Optics Area, Department of Applied Physics, Faculty of Physics, Universitdade de Santiago de Compostela, Santiago de Compostela, Spain.
Front Neurol. 2019 Aug 22;10:911. doi: 10.3389/fneur.2019.00911. eCollection 2019.
Photobiomodulation (PBM) therapy is a promising therapeutic approach for several pathologies, including stroke. The biological effects of PBM for the treatment of cerebral ischemia have previously been explored as a neuroprotective strategy using different light sources, wavelengths, and incident light powers. However, the capability of PBM as a novel alternative therapy to stimulate the recovery of the injured neuronal tissue after ischemic stroke has been poorly explored. The aim of this study was to investigate the low-level light irradiation therapy by using Light Emitting Diodes (LEDs) as potential therapeutic strategy for stroke. The LED photobiomodulation (continuous wave, 830 nm, 0.2-0.6 J/cm) was firstly evaluated at different energy densities in C17.2 immortalized mouse neural progenitor cell lines, in order to observe if this treatment had any effect on cells, in terms of proliferation and viability. Then, the PBM-LED effect (continuous wave, 830 nm, 0.28 J/cm at brain cortex) on long-term recovery (12 weeks) was analyzed in ischemic animal model by means lesion reduction, behavioral deficits, and functional magnetic resonance imaging (fMRI). Analysis of cellular proliferation after PBM was significantly increased (1 mW) in all different exposure times used; however, this effect could not be replicated experimental conditions, as PBM did not show an infarct reduction or functional recovery. Despite the promising therapeutic effect described for PBM, further preclinical studies are necessary to optimize the therapeutic window of this novel therapy, in terms of the mechanism associated to neurorecovery and to reduce the risk of failure in futures clinical trials.
光生物调节（PBM）疗法是一种针对包括中风在内的多种病症的有前景的治疗方法。先前已探索了PBM治疗脑缺血的生物学效应，将其作为一种使用不同光源、波长和入射光功率的神经保护策略。然而，PBM作为一种新型替代疗法来刺激缺血性中风后受损神经元组织恢复的能力尚未得到充分探索。本研究的目的是研究使用发光二极管（LED）进行低水平光照射疗法作为中风的潜在治疗策略。首先在C17.2永生化小鼠神经祖细胞系中以不同能量密度评估LED光生物调节（连续波，830nm，0.2 - 0.6J/cm），以观察这种治疗在增殖和活力方面对细胞是否有任何影响。然后，通过病变减少、行为缺陷和功能磁共振成像（fMRI）在缺血动物模型中分析PBM - LED效应（连续波，830nm，脑皮质处0.28J/cm）对长期恢复（12周）的影响。在所有使用的不同暴露时间下，PBM后细胞增殖分析均显著增加（1mW）；然而，在实验条件下无法重复此效应，因为PBM未显示梗死灶减少或功能恢复。尽管PBM具有所述的有前景的治疗效果，但就与神经恢复相关的机制而言，仍需要进一步的临床前研究来优化这种新疗法的治疗窗口，并降低未来临床试验失败的风险。
