Silva-Andrade Claudia, Martin Alberto J, Garrido Daniel
Laboratorio de Biología de Redes, Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago 8580000, Chile.
Department of Chemical and Bioprocess Engineering, School of Engineering, Pontificia Universidad Católica de Chile, Santiago 7820436, Chile.
Microorganisms. 2022 Jan 20;10(2):213. doi: 10.3390/microorganisms10020213.
strains are rare opportunistic pathogens associated with botulism intoxication. They have been isolated from foods, soil and be carried asymptomatically or cause botulism outbreaks. Is not taxonomically related to , but some strains are equipped with BoNT/F7 cluster. Despite their relationship with diseases, our knowledge regarding the genomic features and phylogenetic characteristics is limited. We analyzed the pangenome of to understand the diversity and genomic features of this species. We compared existing genomes in public databases, metagenomes, and one newly sequenced strain isolated from an asymptomatic subject. The pangenome was open, indicating it comprises genetically diverse organisms. The core genome contained 28.49% of the total genes of the pangenome. Profiling virulence factors confirmed the presence of phospholipase C in some strains, a toxin capable of disrupting eukaryotic cell membranes. Furthermore, the genomic analysis indicated significant horizontal gene transfer (HGT) events as defined by the presence of prophage genomes. Seven strains were equipped with BoNT/F7 cluster. The active site was conserved in all strains, identifying a missing 7-aa region upstream of the active site in genomes. This analysis could be important to advance our knowledge regarding opportunistic clostridia and better understand their contribution to disease.
菌株是与肉毒中毒相关的罕见机会性病原体。它们已从食物、土壤中分离出来，可无症状携带或引发肉毒中毒疫情。它们与[具体物种名称未给出]在分类学上无关联，但一些菌株配备了BoNT/F7簇。尽管它们与疾病有关，但我们对其基因组特征和系统发育特性的了解有限。我们分析了[具体物种名称未给出]的泛基因组，以了解该物种的多样性和基因组特征。我们比较了公共数据库中的现有基因组、宏基因组以及从一名无症状个体中分离出的一个新测序菌株。泛基因组是开放的，表明它包含基因多样的生物体。核心基因组占泛基因组总基因的28.49%。对毒力因子进行分析证实，一些菌株中存在磷脂酶C，这是一种能够破坏真核细胞膜的毒素。此外，基因组分析表明存在由前噬菌体基因组的存在所定义的显著水平基因转移（HGT）事件。七个菌株配备了BoNT/F7簇。活性位点在所有菌株中都是保守的，在[具体物种名称未给出]基因组的活性位点上游发现了一个缺失的7个氨基酸区域。该分析对于增进我们对机会性梭菌的了解以及更好地理解它们对疾病的贡献可能很重要。
