Muema Esther K, Steenkamp Emma T, Venter Stephanus N
DSI-NRF Centre of Excellence in Plant Health Biotechnology, Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South Africa.
Microorganisms. 2022 Jan 20;10(2):216. doi: 10.3390/microorganisms10020216.
Total and diazotrophic bacteria were assessed in the rhizosphere soils of native and encroaching legumes growing in the Succulent Karoo Biome (SKB), South Africa. These were , , , and , of Fabaceae family near Springbok (Northern Cape Province) and neighboring refugia of the Fynbos biome for for comparison purposes. Metabarcoding approach using 16S rRNA gene revealed (26.7%) (23.6%) and (10%), while the gene revealed (70.3%) and (29.5%) of the total sequences recovered as the dominant phyla. Some of the diazotrophs measured were assigned to families; (39%) and (24.4%) (all legumes), (7.9%), (4.6%) and (3%) (, , ) (4.2%; ), (4%; , ), (3.1%; ), and (2.7%; ) of the total sequences recovered. These families have the potential to fix the atmospheric nitrogen. While some diazotrophs were specific or shared across several legumes, a member of species was common in all rhizosphere soils considered. had statistically significantly higher Alpha and distinct Beta-diversity values, than other legumes, supporting its influence on soil microbes. Overall, this work showed diverse bacteria that support plant life in harsh environments such as the SKB, and shows how they are influenced by legumes.
在南非肉质卡鲁生物群落（SKB）中生长的本地和入侵豆科植物的根际土壤中，对总细菌和固氮细菌进行了评估。这些植物分别是生长在斯普林博克（北开普省）附近的豆科植物以及开普植物区系生物群落相邻避难所中的[具体植物名称1]、[具体植物名称2]、[具体植物名称3]和[具体植物名称4]，以作比较。使用16S rRNA基因的宏条形码方法显示，在回收的总序列中，[优势菌门1]占26.7%，[优势菌门2]占23.6%，[优势菌门3]占10%；而使用[另一种基因名称]基因显示，[优势菌门4]占70.3%，[优势菌门5]占29.5%，为主要菌门。所检测到的一些固氮菌被归为不同科；[科名1]（占回收总序列的39%）和[科名2]（占24.4%）（所有豆科植物），[科名3]（占7.9%），[科名4]（占4.6%）和[科名5]（占3%）（[具体植物名称5]、[具体植物名称6]、[具体植物名称7]）（占4.2%；[相关说明]），[科名6]（占4%；[具体植物名称8]、[具体植物名称9]），[科名7]（占3.1%；[具体植物名称10]），以及[科名8]（占2.7%；[具体植物名称11]）。这些科具有固定大气氮的潜力。虽然一些固氮菌在几种豆科植物中具有特异性或共有性，但[某种物种名称]的一个成员在所有所考虑的根际土壤中都很常见。[具体植物名称12]在统计学上具有显著更高的α多样性和独特的β多样性值，高于其他豆科植物，这支持了其对土壤微生物的影响。总体而言，这项工作表明在诸如SKB这样的恶劣环境中存在多种支持植物生长的细菌，并展示了它们如何受到豆科植物的影响。
