Rossi C R, Campos M, Ghanekar S V, Panangala V S
Vet Immunol Immunopathol. 1986 Apr;11(4):319-24. doi: 10.1016/0165-2427(86)90034-6.
Hybridomas to bovine leukocytes were produced by immunization of BALB/C mice with bovine lymphoblasts and fusion of the mouse spleen cells with mouse myeloma cells. Monoclonal antibodies (MABs) were tested against various cell populations by indirect fluorescent microscopy using fluorochrome conjugated antibodies to mouse immunoglobulins. MAB-15, one of the resulting MABs obtained after cloning antibody-producing hybridomas, reacted with 56.8 +/- 8.4% of peripheral blood mononuclear cells (PBMC). MAB-15 did not react with monocytes or B cells, but did react with T cells (fluorescein isothiocyanate-conjugated peanut agglutinin positive cells). MAB-15 reacted with 3.2% of thymocytes from adult cattle. In addition to reacting with T cells, MAB-15 reacted with neutrophils and eosinophils. MAB-15 was characterized as an IgM antibody that was unable to lyse PBMC in the presence of complement. Thus, MAB-15 is a useful marker of mature T cells in the mononuclear cell population.
通过用牛淋巴母细胞免疫BALB/C小鼠，并将小鼠脾细胞与小鼠骨髓瘤细胞融合，制备了针对牛白细胞的杂交瘤。使用荧光素偶联的抗小鼠免疫球蛋白抗体，通过间接荧光显微镜检测单克隆抗体（MABs）对各种细胞群体的反应。MAB-15是在克隆产生抗体的杂交瘤后获得的一种MAB，它与56.8±8.4%的外周血单个核细胞（PBMC）发生反应。MAB-15不与单核细胞或B细胞反应，但与T细胞（异硫氰酸荧光素偶联的花生凝集素阳性细胞）反应。MAB-15与成年牛3.2%的胸腺细胞反应。除了与T细胞反应外，MAB-15还与中性粒细胞和嗜酸性粒细胞反应。MAB-15被鉴定为一种IgM抗体，在补体存在的情况下不能裂解PBMC。因此，MAB-15是单核细胞群体中成熟T细胞的有用标志物。
