Bao Gegen, Zhou Qi, Li Shengyu, Ashraf Umair, Huang Suihua, Miao Aimin, Cheng Zhishang, Wan Xiaorong, Zheng Yixiong
Guangzhou Key Laboratory for Research and Development of Crop Germplasm Resources, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Department of Botany, Division of Science and Technology, University of Education, Lahore, Pakistan.
Front Plant Sci. 2022 Feb 8;12:807021. doi: 10.3389/fpls.2021.807021. eCollection 2021.
Ultrasonic (US) treatment is an efficient method to induce crop tolerance against heavy metal toxicity; however, US-induced aluminum (Al) tolerance in peanuts was rarely studied. This study was comprised of two treatments, namely, CK, without ultrasonic treatment, and US, an ultrasonic seed treatment, for 15 min. Both treated and non-treated treatments were applied with Al in the form of AlCl.18HO at 5 mmol L in Hoagland solution at one leaf stage. Results depicted that plant height, main root length, and number of lateral roots increased significantly under US treatment. Transcriptome analysis revealed that plant hormone signal transduction and transcription factors (TFs) were significantly enriched in the differentially expressed genes (DEGs) in US treatment, and the plant hormones were measured, including salicylic acid (SA) and abscisic acid (ABA) contents, were substantially increased, while indole acetic acid (IAA) and jasmonic acid (JA) contents were decreased significantly in US treatment. The TFs were verified using quantitative real-time (qRT)-PCR, and it was found that multiple TFs genes were significantly upregulated in US treatment, and and genes were also significantly upregulated in US treatment. Overall, the US treatment induced the regulation of hormone content and regulated gene expression by regulating TFs to improve Al tolerance in peanuts. This study provided a theoretical rationale for US treatment to improve Al tolerance in peanuts.
超声波（US）处理是诱导作物对重金属毒性产生耐受性的有效方法；然而，关于超声波诱导花生对铝（Al）耐受性的研究却很少。本研究包括两种处理，即不进行超声波处理的CK和进行15分钟超声波种子处理的US。在一叶期，将处理和未处理的植株均以5 mmol/L的AlCl₃·18H₂O形式施用于Hoagland溶液中的Al。结果表明，超声波处理下植株高度、主根长度和侧根数量显著增加。转录组分析显示，植物激素信号转导和转录因子（TFs）在超声波处理的差异表达基因（DEGs）中显著富集，并且所测定的植物激素包括水杨酸（SA）和脱落酸（ABA）含量大幅增加，而在超声波处理中吲哚乙酸（IAA）和茉莉酸（JA）含量显著降低。使用定量实时（qRT）-PCR对转录因子进行验证，发现多个转录因子基因在超声波处理中显著上调，并且[此处原文似乎缺失具体基因名称]基因在超声波处理中也显著上调。总体而言，超声波处理通过调节转录因子诱导激素含量的调节并调控基因表达，从而提高花生对铝的耐受性。本研究为超声波处理提高花生对铝的耐受性提供了理论依据。
