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的毒力生物标志物：蛋白质组学方法。 （你提供的原文“Virulence Biomarkers of : A Proteomic Approach.”表述不太完整准确，正常可能是“Virulence Biomarkers of [某种病原体之类的] : A Proteomic Approach.” ）

Virulence Biomarkers of : A Proteomic Approach.

作者信息

Cardoso Joana M S, Anjo Sandra I, Manadas Bruno, Silva Hugo, Abrantes Isabel, Nakamura Katsunori, Fonseca Luís

机构信息

Department of Life Sciences, Centre for Functional Ecology, University of Coimbra, Coimbra, Portugal.

CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.

出版信息

Front Plant Sci. 2022 Feb 8;12:822289. doi: 10.3389/fpls.2021.822289. eCollection 2021.

DOI:10.3389/fpls.2021.822289
PMID:35211137
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8861294/
Abstract

The pinewood nematode (PWN), , one of the most serious forest pests worldwide, is considered the causal agent of the pine wilt disease (PWD). The main host species belong to the genus , and a variation in the susceptibility of several pine species to PWN infection is well-known. It is also recognized that there is variation in the virulence among isolates. In the present study, we applied a quantitative mass spectrometry-based proteomics approach to perform a deep characterization of proteomic changes across two isolates with different virulence from different hosts and geographical origins. A total of 1,456 proteins were quantified and compared in the two isolates secretomes, and a total of 2,741 proteins were quantified and compared in the nematode proteomes in pine tree extract and fungus stimuli conditions. From the proteomic analyses, a group of proteins was selected and identified as potential virulence biomarkers and shed light on putative most pathogenic proteins of this plant-parasitic nematode. Proteomic data are available ProteomeXchange with identifier PXD029377.

摘要

松材线虫（PWN）是全球最严重的森林害虫之一，被认为是松材线虫病（PWD）的病原体。主要寄主物种属于松属，几种松树品种对PWN感染的易感性差异是众所周知的。人们还认识到不同分离株之间的毒力存在差异。在本研究中，我们应用了基于定量质谱的蛋白质组学方法，对来自不同寄主和地理来源、具有不同毒力的两个分离株的蛋白质组变化进行深入表征。在两个分离株的分泌蛋白组中总共定量并比较了1456种蛋白质，在松树提取物和真菌刺激条件下的线虫蛋白质组中总共定量并比较了2741种蛋白质。通过蛋白质组分析，选择了一组蛋白质并将其鉴定为潜在的毒力生物标志物，从而揭示了这种植物寄生线虫最具致病性的推定蛋白质。蛋白质组学数据可通过ProteomeXchange获得，标识符为PXD029377。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/2e68ecbaaccd/fpls-12-822289-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/bb94fdfcf0a9/fpls-12-822289-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/9d9576ae3eb5/fpls-12-822289-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/1686d3351960/fpls-12-822289-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/2e68ecbaaccd/fpls-12-822289-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/bb94fdfcf0a9/fpls-12-822289-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/f3d3540f906c/fpls-12-822289-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/df47616c67f7/fpls-12-822289-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/b4d777cdd50c/fpls-12-822289-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/9d9576ae3eb5/fpls-12-822289-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/1686d3351960/fpls-12-822289-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e06/8861294/2e68ecbaaccd/fpls-12-822289-g007.jpg

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