Sakai Yusuke, Wilkens Gerrit D, Wolski Karol, Zapotoczny Szczepan, Heddle Jonathan G
Bionanoscience and Biochemistry Laboratory, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Krakow, Poland.
Postgraduate School of Molecular Medicine, Żwirki I Wigury 61, 02-091 Warsaw, Poland.
ACS Nanosci Au. 2022 Feb 16;2(1):57-63. doi: 10.1021/acsnanoscienceau.1c00027. Epub 2021 Nov 12.
DNA origami is a widely used DNA nanotechnology that allows construction of two-dimensional and three-dimensional nanometric shapes. The designability and rigidity of DNA origami make it an ideal material for construction of topologically linked molecules such as catenanes, which are attractive for their potential as motors and molecular machines. However, a general method for production of topologically linked DNA origami has been lacking. Here, we show that catenated single-stranded DNA circles can be produced and used as a universal scaffold for the production of topologically linked (catenated) DNA origami structures where the individual linked structures can be of any arbitrary design. Assembly of these topologically linked DNA origami structures is achieved via a simple one-pot annealing protocol.
DNA折纸术是一种广泛应用的DNA纳米技术，可用于构建二维和三维纳米级形状。DNA折纸术的可设计性和刚性使其成为构建拓扑连接分子（如连环体）的理想材料，连环体因其作为马达和分子机器的潜力而备受关注。然而，一直缺乏一种生产拓扑连接DNA折纸术的通用方法。在此，我们表明可以生产出连环单链DNA环，并将其用作生产拓扑连接（连环）DNA折纸结构的通用支架，其中各个连接结构可以是任意设计。这些拓扑连接的DNA折纸结构通过简单的一锅退火方案实现组装。
