Wilkens Gerrit, Stepien Piotr, Shaukat Ahmed, Heddle Jonathan
Université de Montpellier, Centre de Biochimie Structurale, CNRS, INSERM, Montpellier, France.
School of Biological and Biomedical Sciences, Durham University, Durham, UK.
Methods Mol Biol. 2025;2901:49-65. doi: 10.1007/978-1-0716-4394-5_5.
DNA origami is a powerful tool for designing and building functional nanoscale machines. There are practical limitations on the size of a single origami meaning that methods to combine multiple DNA origamis into single functional units would be useful. Catenation of DNA origami structures would provide arguably the most stable linkage method, but true catenation requires linking of the DNA origami scaffold strands. This is achieved in the DNA Topogami method, outlined here, which employs a resolvase to produce the scaffold catenanes, which can then be folded into the required structures by staple strands.
DNA折纸术是设计和构建功能性纳米级机器的强大工具。单个折纸的尺寸存在实际限制，这意味着将多个DNA折纸组合成单个功能单元的方法将很有用。DNA折纸结构的连环化可以说是最稳定的连接方法，但真正的连环化需要连接DNA折纸支架链。本文概述的DNA拓扑折纸方法实现了这一点，该方法利用一种解离酶来产生支架连环体，然后可以通过短链将其折叠成所需的结构。
