Nachemson A K, Hansson H A, Dahl D, Lundborg G
Acta Physiol Scand. 1986 Apr;126(4):485-9. doi: 10.1111/j.1748-1716.1986.tb07845.x.
Regeneration of injured sciatic nerve in rats was studied with an immunohistochemical technique visualizing neurofilaments in nerve fibres as well as S-100 protein in Schwann cells. Outgrowing axons with delicate sprouts could be demonstrated along pathways formed by Schwann cells. In contrast, axons which had stopped growing or were degenerating showed bulb-like swellings in their terminal parts. The use of immunohistochemical techniques offers advantages over conventional neurohistochemical staining methods, enabling more detailed observations of nerve regeneration mechanisms in animals.
采用免疫组织化学技术对大鼠坐骨神经损伤后的再生情况进行了研究，该技术可使神经纤维中的神经丝以及雪旺细胞中的S-100蛋白可视化。在雪旺细胞形成的通路中，可以看到有纤细芽突的生长轴突。相比之下，停止生长或正在退化的轴突在其末端部分呈现出球状肿胀。免疫组织化学技术的应用比传统神经组织化学染色方法具有优势，能够更详细地观察动物神经再生机制。
