Department of Dermatology, University Hospital Jena, Jena, Germany.
Dr. Pfleger Arzneimittel GmbH, Bamberg, Germany.
Microbiologyopen. 2022 Feb;11(1):e1257. doi: 10.1002/mbo3.1257.
Dermal fungal infections seem to have increased over recent years. There is further a shift from anthropophilic dermatophytes to a growing prevalence of zoophilic species and the emergence of resistant strains. New antifungals are needed to combat these fungi and their resting spores. This study aimed to investigate the sporicidal effects of sertaconazole nitrate using microplate laser nephelometry against the microconidia of Trichophyton, chlamydospores of Epidermophyton, blastospores of Candida, and conidia of the mold Scopulariopsis brevicaulis. The results obtained were compared with those from ciclopirox olamine and terbinafine. The sporicidal activity was further determined using infected three-dimensional full skin models to determine the antifungal effects in the presence of human cells. Sertaconazole nitrate inhibited the growth of dermatophytes, molds, and yeasts. Ciclopirox olamine also had good antifungal activity, although higher concentrations were needed compared to sertaconazole nitrate. Terbinafine was highly effective against most dermatophytes, but higher concentrations were required to kill the resistant strain Trichophyton indotineae. Sertaconazole nitrate, ciclopirox olamine, and terbinafine had no negative effects on full skin models. Sertaconazole nitrate reduced the growth of fungal and yeast spores over 72 h. Ciclopirox olamine and terbinafine also inhibited the growth of dermatophytes and molds but had significantly lower effects on the yeast. Sertaconazole nitrate might have advantages over the commonly used antifungals ciclopirox olamine and terbinafine in combating resting spores, which persist in the tissues, and thus in the therapy of recurring dermatomycoses.
近年来，皮肤真菌感染似乎有所增加。此外，从亲人性的皮肤真菌向越来越多的亲动物性物种和耐药菌株的流行转变。需要新的抗真菌药物来对抗这些真菌及其休眠孢子。本研究旨在使用微孔板激光散射浊度法研究硝酸舍他康唑对亲人性毛癣菌、亲动物性表皮癣菌的厚壁孢子、亲人性念珠菌的芽生孢子和短帚霉的分生孢子的杀孢子作用。将所得结果与环吡酮胺和特比萘芬进行比较。进一步使用感染的三维全皮肤模型来确定杀孢子活性，以确定在存在人细胞的情况下的抗真菌效果。硝酸舍他康唑抑制皮肤真菌、霉菌和酵母的生长。环吡酮胺也具有良好的抗真菌活性，尽管需要比硝酸舍他康唑更高的浓度。特比萘芬对大多数皮肤真菌都非常有效，但需要更高的浓度才能杀死耐药菌株红色毛癣菌。硝酸舍他康唑、环吡酮胺和特比萘芬对全皮肤模型没有不良影响。硝酸舍他康唑在 72 小时内减少了真菌和酵母孢子的生长。环吡酮胺和特比萘芬也抑制了皮肤真菌和霉菌的生长，但对酵母的抑制作用明显较低。与常用的抗真菌药物环吡酮胺和特比萘芬相比，硝酸舍他康唑在对抗休眠孢子方面可能具有优势，这些休眠孢子在组织中持续存在，因此在复发性皮肤真菌感染的治疗中具有优势。
