从受感染鳞片中分离出DNA后，鉴定出印度ITS基因型VIII的角鲨烯环氧酶基因中的点突变。
Point mutations in the squalene epoxidase gene of Indian ITS genotype VIII identified after DNA isolation from infected scales.
作者信息
Burmester Anke, Hipler Uta-Christina, Hensche Reka, Elsner Peter, Wiegand Cornelia
机构信息
Department of Dermatology, University Hospital Jena, Erfurter Str. 35, D-07743, Jena, Thuringia, Germany.
出版信息
Med Mycol Case Rep. 2019 Sep 18;26:23-24. doi: 10.1016/j.mmcr.2019.09.001. eCollection 2019 Dec.
Terbinafine resistant Indian ITS genotype VIII strain was identified by partial sequencing of the squalene epoxidase gene using DNA isolated from infected scales. This method allowed the rapid identification of single point mutations within the squalene epoxidase gene, even before a fungal culture was obtained. Terbinafine resistance was indicated by the amino acid position switch Phe397Leu based on a single point mutation of the codon changing it from TTC to CTC.
通过使用从感染鳞片中分离的DNA对角鲨烯环氧酶基因进行部分测序，鉴定出对特比萘芬耐药的印度ITS基因型VIII菌株。即使在获得真菌培养物之前，该方法也能快速鉴定角鲨烯环氧酶基因内的单点突变。基于密码子从TTC变为CTC的单点突变，氨基酸位置转换Phe397Leu表明了对特比萘芬的耐药性。
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