Uehlin Andrew F, Vines Jeremy B, Feldman Dale S, Nyairo Elijah, Dean Derrick R, Thomas Vinoy
Department of Materials Science and Engineering, University of Alabama at Birmingham, Birmingham, AL 35205, USA.
Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35205, USA.
Pharmaceutics. 2022 Jan 25;14(2):277. doi: 10.3390/pharmaceutics14020277.
In this study, we report a biohybrid oriented fibrous scaffold based on nanofibers of poly(l-lactic acid) (PLLA)/fibrin produced by electrospinning and subsequent post-treatment. Induced hydrolytic degradation of the fibers in 0.25 M NaOH solution for various time periods followed by the immobilization of fibrin on the hydrolyzed fiber surfaces was shown to significantly affect the mechanical properties, with the tensile strength (40.6 MPa ± 1.3) and strain at failure (38% ± 4.5) attaining a value within the range of human ligaments and ligament-replacement grafts. Unidirectional electrospinning with a mandrel rotational velocity of 26.4 m/s produced highly aligned fibers with an average diameter of 760 ± 96 nm. After a 20-min hydrolysis treatment in NaOH solution, this was further reduced to an average of 457 ± 89 nm, which is within the range of collagen bundles found in ligament tissue. Based on the results presented herein, the authors hypothesize that a combination of fiber orientation/alignment and immobilization of fibrin can result in the mechanical and morphological modification of PLLA tissue scaffolds for ligament-replacement grafts. Further, it was found that treatment with NaOH enhanced the osteogenic differentiation of hMSCs and the additional inclusion of fibrin further enhanced osteogenic differentiation, as demonstrated by decreased proliferative rates and increased ALP activity.
在本研究中，我们报道了一种基于聚（L-乳酸）（PLLA）/纤维蛋白纳米纤维的生物杂交定向纤维支架，该支架通过静电纺丝及后续后处理制备而成。结果表明，将纤维在0.25 M NaOH溶液中进行不同时间段的诱导水解降解，随后在水解后的纤维表面固定纤维蛋白，这会显著影响其力学性能，其拉伸强度（40.6 MPa ± 1.3）和断裂应变（38% ± 4.5）达到了人类韧带及韧带替代移植物的范围内的值。采用26.4 m/s的心轴旋转速度进行单向静电纺丝可产生高度排列的纤维，平均直径为760 ± 96 nm。在NaOH溶液中进行20分钟的水解处理后，平均直径进一步减小至457 ± 89 nm，这在韧带组织中发现的胶原束范围内。基于本文给出的结果，作者推测纤维取向/排列与纤维蛋白固定相结合可导致用于韧带替代移植物的PLLA组织支架的力学和形态学改性。此外，发现用NaOH处理可增强人骨髓间充质干细胞（hMSCs）的成骨分化，而额外加入纤维蛋白进一步增强了成骨分化，这表现为增殖率降低和碱性磷酸酶（ALP）活性增加。
