University of Bologna, Department of Chemistry G. Ciamician and National Consortium of Materials Science and Technology (INSTM, RU Bologna), via Selmi 2, 40126 Bologna, Italy.
J Biomater Sci Polym Ed. 2010;21(10):1283-96. doi: 10.1163/092050609X12517190417597. Epub 2010 Jun 8.
Electrospinning was used to fabricate fibrous scaffolds of lipase-catalyzed poly(omega-pentadecalactone) (PPDL). The slow resorbability of this biomaterial is expected to be valuable for tissue-engineering applications requiring long healing times. The effect of solvent systems and instrumental parameters on fiber morphology was investigated. PPDL electrospinning was optimized and defect-free fibers (diameter 410 +/- 150 nm) were obtained by using a mixed three-solvent system. Scaffolds were characterized by scanning electron microscopy, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and wide angle X-ray diffraction (WAXS). TGA showed no residual solvent in the scaffolds. DSC and WAXS results indicated that electrospun PPDL is semicrystalline. Biocompatibility of PPDL scaffolds was evaluated through indirect cytotoxicity tests using embryonic rat cardiac H9c2 cells. The ability of PPDL electrospun mats to support cell growth was verified by culturing H9c2 cells onto the scaffold. Cell adhesion, proliferation and morphology were evaluated. The results indicated that PPDL mats are not cytotoxic and they support proliferation of H9c2 cells. The cumulative results of this study suggest further exploration of PPDL fibrous mats as scaffolds for tissue-engineered constructs.
静电纺丝被用于制备脂肪酶催化的聚（ω-十五内酯）（PPDL）纤维支架。这种生物材料的缓慢可吸收性有望在需要长时间愈合时间的组织工程应用中具有价值。研究了溶剂体系和仪器参数对纤维形态的影响。通过使用混合三溶剂体系，优化了 PPDL 的静电纺丝工艺，得到了无缺陷的纤维（直径 410±150nm）。通过扫描电子显微镜、热重分析（TGA）、差示扫描量热法（DSC）和广角 X 射线衍射（WAXS）对支架进行了表征。TGA 表明支架中没有残留溶剂。DSC 和 WAXS 结果表明，静电纺丝的 PPDL 是半结晶的。通过使用胚胎大鼠心脏 H9c2 细胞进行间接细胞毒性试验评估了 PPDL 支架的生物相容性。通过将 H9c2 细胞培养在支架上，验证了 PPDL 电纺毡支持细胞生长的能力。评估了细胞黏附、增殖和形态。结果表明，PPDL 毡无细胞毒性，并支持 H9c2 细胞的增殖。这项研究的综合结果表明，进一步探索 PPDL 纤维毡作为组织工程构建物的支架。
