Chen Guan-Rong, Chang Mei-Ling, Chang Shang-Tzen, Ho Yu-Tung, Chang Hui-Ting
School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan.
Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taipei 104, Taiwan.
Pharmaceutics. 2022 Feb 2;14(2):351. doi: 10.3390/pharmaceutics14020351.
The objective of the present study is to evaluate the cytotoxicity of essential oil and its phytochemical on the Hep G2 cell line (human hepatocellular carcinoma). Bark essential oil has significant cytotoxicity to Hep G2 cells, and S3 fraction is the most active fraction in cytotoxicity to Hep G2 cells among the six fractions. The diterpenoid quinone, 6,7-dehydroroyleanone, was isolated from the active S3 fraction by bioassay-guided isolation. 6,7-Dehydroroyleanone exhibited significant cytotoxicity in Hep G2 cells, and the efficacy of 6,7-dehydroroyleanone was better than the positive control, etoposide. Apoptosis analysis of Hep G2 cells with different treatments was characterized via flow cytometry to confirm the cell death situation. Etoposide and 6,7-dehydroroyleanone could induce the apoptosis in Hep G2 cells using flow cytometric assay. Results revealed 6,7-dehydroroyleanone from bark essential oil can be a potential phytochemical to develop the anticancer chemotherapeutic agent for the treatment of the human hepatocellular carcinoma.
本研究的目的是评估香精油及其植物化学成分对Hep G2细胞系（人肝癌细胞）的细胞毒性。树皮香精油对Hep G2细胞具有显著的细胞毒性，并且在六个组分中，S3组分对Hep G2细胞的细胞毒性最为活跃。通过生物活性导向分离从活性S3组分中分离出二萜醌6,7-脱氢罗勒酮。6,7-脱氢罗勒酮在Hep G2细胞中表现出显著的细胞毒性，其效果优于阳性对照依托泊苷。通过流式细胞术对不同处理的Hep G2细胞进行凋亡分析，以确认细胞死亡情况。依托泊苷和6,7-脱氢罗勒酮可通过流式细胞术检测诱导Hep G2细胞凋亡。结果表明，树皮香精油中的6,7-脱氢罗勒酮可能是一种潜在的植物化学成分，可用于开发治疗人类肝细胞癌的抗癌化疗药物。
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