School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan.
Instrumentation Center, College of Science, National Taiwan University, Taipei 106, Taiwan.
Molecules. 2022 Mar 25;27(7):2136. doi: 10.3390/molecules27072136.
(Kanehira) Masamune is a broad-leaved species widespread in East Asia; the wood extract and its constituents possess antifungal activity against wood-decay fungi. Antifungal activities of leaf essential oil and its constituents from were investigated in the present study. Bioassay-guided isolation was applied to isolate the phytochemicals from leaf essential oil. 1D and 2D NMR, FTIR, and MS spectroscopic analyses were applied to elucidate the chemical structures of isolated compounds. Leaf essential oil displayed antifungal activity against wood decay fungi and was further separated into 11 fractions by column chromatography. Four sesquiterpenoids were isolated and identified from the active fractions of leaf essential oil through bioassay-guided isolation. Among these sesquiterpenoids, guaiol, bulnesol, and β-elemol have higher antifungal activity against brown-rot fungus and white-rot fungus . Leaf essential oil and active compounds showed better antifungal activity against than against . The molecular structure of active sesquiterpenoids all contain the hydroxyisopropyl group. Antifungal sesquiterpenoids from leaf essential oil show potential as natural fungicides for decay control of lignocellulosic materials.
(Kanehira) Masamune 是一种广泛分布于东亚的阔叶树种；木材提取物及其成分具有抗真菌活性，可对抗木材腐朽真菌。本研究调查了 叶片精油及其成分的抗真菌活性。采用生物测定指导分离法从叶片精油中分离出植物化学物质。应用 1D 和 2D NMR、FTIR 和 MS 光谱分析阐明了分离化合物的化学结构。叶片精油对木材腐朽真菌具有抗真菌活性，并通过柱层析进一步分离成 11 个馏分。通过生物测定指导分离，从叶片精油的活性馏分中分离并鉴定出 4 种倍半萜。在这些倍半萜中，愈创木醇、对伞花烃和 β-石竹烯对褐腐菌 和白腐菌 的抗真菌活性更高。叶片精油和活性化合物对 显示出比 更好的抗真菌活性。活性倍半萜的分子结构都含有羟基异丙基。来自 叶片精油的抗真菌倍半萜有望成为木质纤维素材料腐朽控制的天然杀菌剂。
