3D Imaging and Display Laboratory, Department of Optics, Universidad de Valencia, 46100 Burjassot, Spain.
School of Physics, Universidad Nacional de Colombia, Medellin 050034, Colombia.
Sensors (Basel). 2022 Feb 14;22(4):1459. doi: 10.3390/s22041459.
In this work, the design, building, and testing of the most portable, easy-to-build, robust, handheld, and cost-effective Fourier Lightfield Microscope (FLMic) to date is reported. The FLMic is built by means of a surveillance camera lens and additional off-the-shelf optical elements, resulting in a cost-effective FLMic exhibiting all the regular sought features in lightfield microscopy, such as refocusing and gathering 3D information of samples by means of a single-shot approach. The proposed FLMic features reduced dimensions and light weight, which, combined with its low cost, turn the presented FLMic into a strong candidate for in-field application where 3D imaging capabilities are pursued. The use of cost-effective optical elements has a relatively low impact on the optical performance, regarding the figures dictated by the theory, while its price can be at least 100 times lower than that of a regular FLMic. The system operability is tested in both bright-field and fluorescent modes by imaging a resolution target, a honeybee wing, and a knot of dyed cotton fibers.
本文报告了迄今为止最便携、易于构建、坚固、手持式且具有成本效益的傅里叶光场显微镜 (FLMic) 的设计、构建和测试。FLMic 通过监控摄像头镜头和其他现成的光学元件构建而成，构成了一个具有成本效益的 FLMic，展示了光场显微镜中所有常规的目标特征，例如单次拍摄即可重新聚焦和收集样本的 3D 信息。所提出的 FLMic 具有更小的尺寸和更轻的重量，结合其低成本，使得所提出的 FLMic 成为在需要 3D 成像能力的现场应用中的有力候选者。使用具有成本效益的光学元件对光学性能的影响相对较小，与理论规定的数值相比，而其价格至少可以比常规的 FLMic 低 100 倍。该系统的操作性通过对分辨率靶标、蜜蜂翅膀和染色棉纤维结进行成像，在明场和荧光两种模式下进行了测试。
