3D Imaging and Display Laboratory, Department of Optics, Universitat de València, 46100 Burjassot, Spain.
Doitplenoptic S.L., 46980 Paterna, Spain.
Sensors (Basel). 2021 Oct 5;21(19):6619. doi: 10.3390/s21196619.
Lightfield microscopy has raised growing interest in the last few years. Its ability to get three-dimensional information about the sample in a single shot makes it suitable for many applications in which time resolution is fundamental. In this paper we present a novel device, which is capable of converting any conventional microscope into a lightfield microscope. Based on the Fourier integral microscope concept, we designed the lightfield microscope eyepiece. This is coupled to the eyepiece port, to let the user exploit all the host microscope's components (objective turret, illumination systems, translation stage, etc.) and get a 3D reconstruction of the sample. After the optical design, a proof-of-concept device was built with off-the-shelf optomechanical components. Here, its optical performances are demonstrated, which show good matching with the theoretical ones. Then, the pictures of different samples taken with the lightfield eyepiece are shown, along with the corresponding reconstructions. We demonstrated the functioning of the lightfield eyepiece and lay the foundation for the development of a commercial device that works with any microscope.
光场显微镜在过去几年中引起了越来越多的关注。它能够在单次拍摄中获取关于样本的三维信息，因此非常适合许多时间分辨率至关重要的应用。在本文中，我们提出了一种新颖的装置，能够将任何传统显微镜转换为光场显微镜。基于傅里叶积分显微镜的概念，我们设计了光场显微镜目镜。它与目镜端口耦合，让用户利用显微镜的所有主机组件（物镜转塔、照明系统、平移台等），并对样本进行三维重建。在进行光学设计后，我们使用现成的光电机械组件构建了一个概念验证设备。在此，展示了其光学性能，与理论性能匹配良好。然后，展示了用光场目镜拍摄的不同样本的图片及其相应的重建。我们演示了光场目镜的功能，并为开发可与任何显微镜配合使用的商用设备奠定了基础。
