Gallus Roberto, Gheit Tarik, Holzinger Dana, Petrillo Marco, Rizzo Davide, Petrone Gianluigi, Miccichè Francesco, Mattiucci Gian Carlo, Arciuolo Damiano, Capobianco Giampiero, Delogu Giovanni, Valentini Vincenzo, Tommasino Massimo, Bussu Francesco
Otolaryngology, Mater Olbia Hospital, 07026 Olbia, Italy.
Infections and Cancer Biology Group, International Agency for Research on Cancer, World Health Organization, 69008 Lyon, France.
Vaccines (Basel). 2022 Jan 27;10(2):204. doi: 10.3390/vaccines10020204.
The mucosal high-risk (HR) human papillomavirus (HPV) is associated with oropharyngeal carcinogenesis. Aims of this study were to evaluate the prevalence of HR-HPV infection in laryngeal squamous cell carcinoma (LSCC) from different subsites, and the clinico-biological meaning of p16 overexpression. Ninety-seven LSCCs submitted to primary surgery ( = 75) or to post-irradiation salvage laryngectomy ( = 22) were evaluated for HR-HPV DNA and RNA using Luminex-based assays. p16 immunohistochemistry was performed. HR-HPV DNA from HPV16 was detected in seven cases (8.75%), without significant differences between supraglottic and glottic lesions. HPV RNA was never detected. p16 overexpression correlated with HR-HPV DNA, but the kappa agreement score was poor. HPV DNA showed no impact on prognosis. p16 overexpression was associated with a better survival (OS, RFS) in primarily operated cases, while an inverse association with OS was observed in the salvage surgery group. HR-HPV infection appears to have a marginal role in LSCC independent of the anatomical subsite. p16 expression is deregulated in LSCC independent of HPV but displays a prognostic role in patients submitted to primary surgery. The negative predictive role of p16 overexpression in patients undergoing salvage surgery deserves more investigations for validation and elucidation of its clinical relevance.
黏膜高危（HR）人乳头瘤病毒（HPV）与口咽癌发生相关。本研究旨在评估不同亚部位喉鳞状细胞癌（LSCC）中HR-HPV感染的患病率以及p16过表达的临床生物学意义。使用基于Luminex的检测方法对97例接受初次手术（n = 75）或放疗后挽救性喉切除术（n = 22）的LSCC患者进行HR-HPV DNA和RNA检测，并进行p16免疫组化。在7例病例（8.75%）中检测到来自HPV16的HR-HPV DNA，声门上和声门病变之间无显著差异。未检测到HPV RNA。p16过表达与HR-HPV DNA相关，但kappa一致性评分较差。HPV DNA对预后无影响。在初次手术病例中，p16过表达与较好的生存率（总生存期、无复发生存期）相关，而在挽救性手术组中观察到与总生存期呈负相关。HR-HPV感染在LSCC中似乎起边缘作用，与解剖亚部位无关。p16表达在LSCC中不受HPV影响而失调，但在接受初次手术的患者中具有预后作用。p16过表达在接受挽救性手术患者中的阴性预测作用值得进一步研究以验证并阐明其临床相关性。
