Serra Leo, Tan Sovanna, Robinson Sarah, Langdale Jane A
The Sainsbury Laboratory, University of Cambridge, Cambridge CB2 1LR, UK.
Department of Plant Sciences, University of Oxford, South Parks Rd., Oxford OX1 3RB, UK.
Plants (Basel). 2022 Feb 13;11(4):506. doi: 10.3390/plants11040506.
Plant development is a complex process that relies on molecular and cellular events being co-ordinated in space and time. Microscopy is one of the most powerful tools available to investigate this spatiotemporal complexity. One step towards a better understanding of complexity in plants would be the acquisition of 3D images of entire organs. However, 3D imaging of intact plant samples is not always simple and often requires expensive and/or non-trivial approaches. In particular, the inner tissues of thick samples are challenging to image. Here, we present the Flip-Flap method, a simple imaging protocol to produce 3D images of cleared plant samples at the organ scale. This method allows full 3D reconstruction of plant organs suitable for 3D segmentation and further related analysis and can be easily handled by relatively inexperienced microscopists.
植物发育是一个复杂的过程，它依赖于分子和细胞事件在空间和时间上的协调。显微镜检查是研究这种时空复杂性最强大的工具之一。朝着更好地理解植物复杂性迈出的一步是获取整个器官的三维图像。然而，完整植物样本的三维成像并不总是简单的，而且通常需要昂贵和/或复杂的方法。特别是，厚样本的内部组织成像具有挑战性。在这里，我们介绍了翻转法，这是一种简单的成像方案，用于在器官尺度上生成透明植物样本的三维图像。该方法允许对适合三维分割及进一步相关分析的植物器官进行完整的三维重建，并且相对缺乏经验的显微镜工作者也可以轻松操作。
