James Tymon, Johnson Alexandra, Schaller Alexander, Vanderzande Stijn, Luo Feixiong, Sandefur Paul, Ru Sushan, Peace Cameron
Department of Horticulture, Washington State University, Pullman, WA 99164, USA.
Department of Environmental Horticulture, University of Florida, Gainesville, FL 32611, USA.
Plants (Basel). 2022 Feb 14;11(4):517. doi: 10.3390/plants11040517.
Providing hands-on education for the next generation of plant breeders would help maximize effectiveness of future breeding efforts. Such education should include training in introgression of crop wild relative alleles, which can increase genetic diversity while providing cultivar attributes that meet industry and consumer demands in a crop such as cider apple. Incorporation of DNA information in breeding decisions has become more common and is another skill future plant breeders need. The Palouse Wild Cider apple breeding program (PWCabp) was established at Washington State University in early 2014 as a student-run experiential learning opportunity. The objectives of this study were to describe the PWCabp's approaches, outcomes, and student involvement to date that has relied on a systematic operational structure, utilization of wild relatives, and incorporation of DNA information. Students chose the crop (cider apple) and initial target market and stakeholders (backyard growers and hobbyists of the Palouse region). Twelve target attributes were defined including high phenolics and red flesh. Phase one and two field trials were established. Two promising high-bitterness selections were identified and propagated. By running the PWCabp, more than 20 undergraduate and graduate students gained experience in the decisions and operations of a fruit breeding program. PWCabp activities have produced desirable new germplasm via utilization of highly diverse germplasm and trained new plant breeding professionals via experiential learning.
为下一代植物育种者提供实践教育将有助于最大限度地提高未来育种工作的成效。此类教育应包括作物野生近缘种等位基因渐渗方面的培训,这可以增加遗传多样性,同时提供满足苹果酒用苹果等作物行业和消费者需求的品种特性。在育种决策中纳入DNA信息已变得更加普遍,这是未来植物育种者所需的另一项技能。帕卢斯野生苹果酒用苹果育种项目(PWCabp)于2014年初在华盛顿州立大学设立,是一个由学生主导的实践学习机会。本研究的目的是描述PWCabp迄今为止所采用的方法、成果以及学生参与情况,该项目依赖于系统的运作结构、野生近缘种的利用以及DNA信息的纳入。学生们选择了作物(苹果酒用苹果)以及初始目标市场和利益相关者(帕卢斯地区的后院种植者和业余爱好者)。确定了12个目标特性,包括高酚类物质含量和红肉。建立了第一阶段和第二阶段的田间试验。鉴定并繁殖出了两个有前景的高苦味选择品种。通过开展PWCabp,20多名本科生和研究生在水果育种项目的决策和运作方面积累了经验。PWCabp的活动通过利用高度多样化的种质产生了理想的新种质,并通过实践学习培养了新的植物育种专业人员。