Munda Sunita, Saikia Raktim Jyoti, Begum Twahira, Bhandari Sangeeta, Gogoi Ankita, Sarma Neelav, Tamang Raghu, Lal Mohan
CSIR-North East Institute of Science and Technology, Jorhat 785006, Assam, India.
AcSIR-Academy of Scientific and Innovative Research, Ghaziabad 201002, Uttar Pradesh, India.
Plants (Basel). 2022 Feb 16;11(4):528. doi: 10.3390/plants11040528.
Jowitt is an industrially important crop due to its value in the aromatic, perfumery and pharmaceutical industries. In this study, 72 accessions of were selected for molecular diversity analysis using SSR markers. It revealed a total of 65 polymorphic alleles showing an average of 68.10% polymorphism. The best SSR primer with competency in discriminating the germplasm was 3CM0506 with PIC (0.69), MI (0.69) and Rp (3.12). Genetic variation was studied between Assam, Manipur, Meghalaya and Arunachal Pradesh populations. A dendrogram based on the Neighbour-Joining Method showed clustering of germplasm on the collection site. A total of six relevant genetic populations were identified through a structure harvester software analysis. Moreover, a dendrogram based on similarity, complete linkage and Euclidean distance was also elucidated differentiating the genotypes with respect to the major phytochemical constituents of the essential oil. GC-FID and GC-MS analyses of the essential oil of the 72 germplasms revealed citronellal content from 2.58-51.45%, citronellol from 0.00-26.39% and geraniol from 0.00-41.15%. This is the first molecular diversity report with 72 accessions of collected from the NE region using 28 SSR primers as well as their diversity based on phytochemical markers. This diversity computation will help with acquisition of the knowledge and relationship among each individual accession leading to the development of improved and essential oil component-rich cultivars.
由于在香料、香水和制药行业的价值，乔伊特是一种具有重要工业价值的作物。在本研究中，选择了72份该作物种质用于SSR标记的分子多样性分析。结果显示共有65个多态性等位基因，平均多态性为68.10%。在鉴别种质方面表现最佳的SSR引物是3CM0506，其PIC为0.69、MI为0.69、Rp为3.12。对阿萨姆邦、曼尼普尔邦、梅加拉亚邦和阿鲁纳恰尔邦的种群进行了遗传变异研究。基于邻接法的树形图显示种质在收集地点上的聚类情况。通过结构收割机软件分析共鉴定出6个相关遗传种群。此外，还绘制了基于相似性、完全连锁和欧氏距离的树形图，以区分不同基因型在精油主要植物化学成分方面的差异。对72种种质的精油进行GC-FID和GC-MS分析，结果显示香茅醛含量为2.58 - 51.45%，香茅醇含量为0.00 - 26.39%，香叶醇含量为0.00 - 41.15%。这是首次使用28个SSR引物对从东北地区收集的72份该作物种质进行分子多样性报告，以及基于植物化学标记的多样性研究。这种多样性计算将有助于获取各个种质之间的知识和关系，从而培育出改良的、富含精油成分的品种。
