Bishoyi Ashok Kumar, Sharma Anjali, Kavane Aarti, Geetha K A
PDPIAS, Charotar University of Science and Technology, Changa, Anand, Gujarat, India.
ICAR-Directorate of Medicinal and Aromatic Plants Research, Boriavi, Anand, Gujarat, India.
Appl Biochem Biotechnol. 2016 Jun;179(4):659-70. doi: 10.1007/s12010-016-2022-y. Epub 2016 Feb 27.
Cymbopogon is an important genus of family Poaceae, cultivated mainly for its essential oils which possess high medicinal and economical value. Several cultivars of Cymbopogon species are available for commercial cultivation in India and identification of these cultivars was conceded by means of morphological markers and essential oil constitution. Since these parameters are highly influenced by environmental factors, in most of the cases, it is difficult to identify Cymbopogon cultivars. In the present study, Random amplified polymorphic DNA (RAPD) and Inter-simple sequence repeat (ISSR) markers were employed to discriminate nine leading varieties of Cymbopogon since prior genomic information is lacking or very little in the genus. Ninety RAPD and 70 ISSR primers were used which generated 63 and 69 % polymorphic amplicons, respectively. Similarity in the pattern of UPGMA-derived dendrogram of RAPD and ISSR analysis revealed the reliability of the markers chosen for the study. Varietal/cultivar-specific markers generated from the study could be utilised for varietal/cultivar authentication, thus monitoring the quality of the essential oil production in Cymbopogon. These markers can also be utilised for the IPR protection of the cultivars. Moreover, the study provides molecular marker tool kit in both random and simple sequence repeats for diverse molecular research in the same or related genera.
香茅属是禾本科的一个重要属，主要因其具有高药用和经济价值的精油而被种植。在印度，有几种香茅属品种可供商业种植，这些品种通过形态学标记和精油成分来鉴定。由于这些参数受环境因素的影响很大，在大多数情况下，很难鉴定香茅属品种。在本研究中，由于该属缺乏或仅有极少的先前基因组信息，因此采用随机扩增多态性DNA（RAPD）和简单序列重复区间（ISSR）标记来区分9个主要的香茅品种。使用了90个RAPD引物和70个ISSR引物，分别产生了63%和69%的多态性扩增子。RAPD和ISSR分析的基于非加权组平均法（UPGMA）的树状图模式的相似性揭示了本研究中所选标记的可靠性。本研究产生的品种/栽培品种特异性标记可用于品种/栽培品种鉴定，从而监测香茅精油生产的质量。这些标记还可用于品种的知识产权保护。此外，本研究为同一属或相关属的各种分子研究提供了随机和简单序列重复的分子标记工具包。
