Saini Komal, Modgill Nancy, Singh Kamalinder K, Kakkar Vandita
Department of Pharmaceutics, University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh 160014, India.
School of Pharmacy and Biomedical Sciences, Faculty of Clinical and Biomedical Sciences, University of Central Lancashire, Preston PR1 2HE, UK.
Nanomaterials (Basel). 2022 Feb 14;12(4):636. doi: 10.3390/nano12040636.
Treatment of atopic dermatitis (AD) is challenging due to its complex pathophysiology. Tetrahydrocurcumin (THC) a polyphenolic, colorless compound that is more polar than curcumin. It possesses superior anti-inflammatory properties and has a clinical advantage over curcumin. The present study investigated the therapeutic effectiveness of THC solid lipid nanoparticle (THC-SLN)-based gels in AD. THC-SLNs prepared using microemulsification resulted in a particle size of 109.2 nm as determined by nanoparticle tracking, and FTIR confirmed the entrapment of drug within the lipid matrix. THC-SLNs greatly enhanced skin hydration when tested both ex vivo and in vivo in Lacca mice. Deeper skin penetration was clearly established using dermatokinetics and CLSM. The in vivo pharmacodynamics of THC-SLNs gel in 2,4-dinitrochlorobenzene (DNCB)-induced AD mice showed enhanced bioactivity; reduced levels of TNF-α and IL-6; and complete healing, as evident from histopathological studies. Thus, the novel topical THC-SLN gel has potential to emerge as a safe alternative to conventional corticosteroids for AD and other skin disorders with overbearing inflammation.
由于特应性皮炎（AD）复杂的病理生理学，其治疗具有挑战性。四氢姜黄素（THC）是一种多酚类无色化合物，比姜黄素极性更强。它具有卓越的抗炎特性，与姜黄素相比具有临床优势。本研究调查了基于四氢姜黄素固体脂质纳米粒（THC-SLN）的凝胶在特应性皮炎中的治疗效果。使用微乳化法制备的THC-SLN通过纳米颗粒跟踪测定粒径为109.2nm，傅里叶变换红外光谱（FTIR）证实药物被包裹在脂质基质中。在紫胶小鼠体内和体外测试时，THC-SLN均能显著增强皮肤水合作用。通过皮肤动力学和共聚焦激光扫描显微镜（CLSM）明确证实了其更深的皮肤渗透。THC-SLN凝胶在2,4-二硝基氯苯（DNCB）诱导的特应性皮炎小鼠体内的药效学研究显示出增强的生物活性；肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）水平降低；组织病理学研究表明完全愈合。因此，新型局部用THC-SLN凝胶有可能成为治疗特应性皮炎和其他伴有严重炎症的皮肤疾病的传统皮质类固醇的安全替代品。
