体重减轻和短链脂肪酸可减少肥胖患者单核细胞和脂肪组织巨噬细胞的全身炎症。

Weight Loss and Short-Chain Fatty Acids Reduce Systemic Inflammation in Monocytes and Adipose Tissue Macrophages from Obese Subjects.

机构信息

Priority Research Centre for Healthy Lungs, Hunter Medical Research Institute, University of Newcastle, New Lambton Heights, Newcastle, NSW 2305, Australia.

Department of Surgery, John Hunter Hospital, New Lambton Heights, Newcastle, NSW 2305, Australia.

出版信息

Nutrients. 2022 Feb 11;14(4):765. doi: 10.3390/nu14040765.

BACKGROUND

Chronic low-grade systemic inflammation is a characteristic of obesity that leads to various non-communicable diseases. Weight loss and SCFAs are potential strategies for attenuating obese systemic inflammation.

METHODS

Blood samples were collected from 43 obese subjects (BMI ≥ 30 kg/m) scheduled for laparoscopic bariatric sleeve surgery, 26 obese subjects at follow-up 12-18 months post-surgery and 8 healthy weight subjects (BMI 18.5-24.9 kg/m). Monocytes were isolated from blood and adipose tissue macrophages from visceral adipose tissue of obese subjects only. Isolated cells stimulated with 1 ng/mL LPS and treated simultaneously with 300 mM of sodium acetate or 30 mM of sodium propionate or butyrate and supernatant were harvested after 15 h incubation. TNF-α and IL-6 cytokines were measured via ELISA and mRNA gene expression of and , , and , and and via RT-qPCR.

RESULTS

TNF-α and IL-6 production and and mRNA expression were significantly decreased in follow-up subjects compared to baseline. SCFAs significantly reduced TNF-α and IL-6 and altered and mRNA expression in monocytes and macrophages from obese subjects.

CONCLUSION

Weight loss and ex vivo SCFA treatments were successful in combatting systemic inflammation in obesity. Results highlighted molecular changes that occur with weight loss and as a result of SCFA treatment.

背景

慢性低度全身炎症是肥胖的特征，可导致各种非传染性疾病。减肥和短链脂肪酸（SCFAs）是减轻肥胖全身炎症的潜在策略。

方法

从计划接受腹腔镜胃袖状切除术的 43 名肥胖受试者（BMI≥30kg/m²）、26 名术后 12-18 个月随访的肥胖受试者和 8 名健康体重受试者（BMI 18.5-24.9kg/m²）采集血样。仅从肥胖受试者的内脏脂肪组织中分离血液中的单核细胞和脂肪组织巨噬细胞。分离的细胞用 1ng/ml LPS 刺激，并同时用 300mm 醋酸钠或 30mm 丙酸钠或丁酸钠处理，孵育 15 小时后收获上清液。通过 ELISA 测量 TNF-α 和 IL-6 细胞因子，并通过 RT-qPCR 测量和、和、和、和的 mRNA 基因表达。