Research Center for Bioconvergence Analysis, Korea Basic Science Institute (KBSI), Ochang 28119, Korea.
Bio-Analytical Science Division, University of Science and Technology (UST), Daejeon 34113, Korea.
Int J Mol Sci. 2022 Feb 16;23(4):2187. doi: 10.3390/ijms23042187.
Rapid and precise diagnostic methods are required to control emerging infectious diseases effectively. Human body fluids are attractive clinical samples for discovering diagnostic targets because they reflect the clinical statuses of patients and most of them can be obtained with minimally invasive sampling processes. Body fluids are good reservoirs for infectious parasites, bacteria, and viruses. Therefore, recent clinical proteomics methods have focused on body fluids when aiming to discover human- or pathogen-originated diagnostic markers. Cutting-edge liquid chromatography-mass spectrometry (LC-MS)-based proteomics has been applied in this regard; it is considered one of the most sensitive and specific proteomics approaches. Here, the clinical characteristics of each body fluid, recent tandem mass spectroscopy (MS/MS) data-acquisition methods, and applications of body fluids for proteomics regarding infectious diseases (including the coronavirus disease of 2019 [COVID-19]), are summarized and discussed.
需要快速准确的诊断方法来有效控制新发传染病。人体体液是发现诊断靶点的有吸引力的临床样本，因为它们反映了患者的临床状况，而且大多数可以通过微创取样过程获得。体液是传染性寄生虫、细菌和病毒的良好储存库。因此，最近的临床蛋白质组学方法在发现源自人类或病原体的诊断标志物时，主要集中在体液上。基于液相色谱-质谱（LC-MS）的前沿蛋白质组学已应用于这方面；它被认为是最敏感和最特异的蛋白质组学方法之一。在这里，总结和讨论了每种体液的临床特征、最近的串联质谱（MS/MS）数据采集方法，以及体液在传染病（包括 2019 年冠状病毒病[COVID-19]）方面的蛋白质组学应用。
