Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
Proc Natl Acad Sci U S A. 2022 Mar 1;119(9). doi: 10.1073/pnas.2026355119.
Seed vigor in crops is important in terms of improving grain quality and germplasm conservation; however, little is known about its regulatory mechanisms through the encoded proteome and gene network. Comparative analyses of transcriptome (RNA sequencing [RNA-seq]) and broadly targeted metabolic profiling of two subspecific rice cultivars with distinct seed vigor during accelerated aging revealed various biological pathways and metabolic processes as key influences explaining trait differences. RNA-seq coexpression regulatory network analyses identified several transcription factors, including bZIP23 and bZIP42, that act as nodes in the gene network. Importantly, transgenic seeds of overexpression of enhanced seed vigor, whereas its gene knockout reduced seed vigor, suggesting that the protein it encodes functions as a positive regulator. Similarly, overexpression and knockout of that encodes a key player in the detoxification pathway enhanced and decreased seed vigor, respectively. We further demonstrated a direct interaction of the promoter with bZIP23 in seeds, which activates the expression of , and the genetic evidence suggested that most likely functions in a common pathway with and acts upstream of to modulate seed vigor. In addition, the control of seed vigor by the bZIP23-PER1A module was connected with that of the abscisic acid signaling pathway. Collectively, we revealed the genetic architecture of variation in seed vigor and uncovered the bZIP23-PER1A-mediated detoxification pathway that enhances the trait in rice.
作物种子活力在提高粮食质量和种质资源保护方面很重要；然而，其通过编码蛋白质组和基因网络的调控机制知之甚少。对两个亚种水稻品种在加速老化过程中具有不同种子活力的转录组（RNA 测序 [RNA-seq]）和广泛靶向代谢组学的比较分析，揭示了各种生物途径和代谢过程是解释性状差异的关键影响因素。RNA-seq 共表达调控网络分析鉴定了几个转录因子，包括 bZIP23 和 bZIP42，它们作为基因网络中的节点发挥作用。重要的是，过表达增强种子活力，而其基因敲除则降低种子活力，表明其编码的蛋白质作为正调控因子发挥作用。同样，在解毒途径中发挥关键作用的基因的过表达和敲除分别增强和降低了种子活力。我们进一步证明了该基因的启动子与种子中的 bZIP23 直接相互作用，激活了该基因的表达，遗传证据表明该基因可能与和一起在一个共同途径中发挥作用，调节种子活力。此外，bZIP23-PER1A 模块对种子活力的控制与脱落酸信号通路的控制有关。总的来说，我们揭示了种子活力变异的遗传结构，并揭示了 bZIP23-PER1A 介导的解毒途径增强了水稻的这一特性。
