Fletcher J A, Bell R, Koekebakker M, Dowers A A, McCaffrey R P, Barr R D
Blood. 1986 Jul;68(1):310-2.
Expression of terminal transferase (TdT) is believed to be restricted to primitive lymphoid cells; recently, however, indirect immunofluorescent (IF) assays have been used to demonstrate the apparent presence of TdT in phytohemagglutinin (PHA)-stimulated peripheral blood lymphocytes and in various nonlymphoid malignancies. Using an IF assay, we found that a heteroantiserum to TdT reacted with cultured and PHA-stimulated human peripheral blood mononuclear cells, but we were unable to confirm the presence of TdT in these cells using immunoblotting and biochemical assays. We conclude that the IF results are spurious and most likely represent recognition by the heteroantiserum of inducible protein(s) other than TdT.
末端转移酶（TdT）的表达被认为仅限于原始淋巴细胞；然而，最近间接免疫荧光（IF）检测已被用于证明在植物血凝素（PHA）刺激的外周血淋巴细胞和各种非淋巴恶性肿瘤中明显存在TdT。使用IF检测，我们发现抗TdT异源抗血清与培养的和PHA刺激的人外周血单个核细胞发生反应，但我们无法通过免疫印迹和生化检测来证实这些细胞中存在TdT。我们得出结论，IF结果是虚假的，很可能代表异源抗血清识别了TdT以外的诱导蛋白。
