Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, 600 119, India.
Centre of Advanced Study (CAS) in Marine Biology, Annamalai University, Parangipettai, Cuddalore, Tamil Nadu, 632502, India.
Sci Rep. 2022 Feb 25;12(1):3217. doi: 10.1038/s41598-022-05779-7.
The cultural microbiomes of 27 bacteria colonies were isolated from Mugil cephalus for analysis of the antibacterial and antagonistic activities. A potent probiotic bacterium was characterized using16S r RNA sequencing. The potent strain was added to fish diet to perform the challenge test and to study the growth and immunological parameter. The extracellular proteins from the probiotic were collected and characterized using MALDI TOF/TOF. Out of G27, G9 strain inhibited all the five pathogenic strains. An isolated bacterium was identified as Bacillus subtilis PRBD09 with accession number KF765648. After 35 days of feeding period B. subtilis PRBD09 enhance the both cellular and humoral immune responses, which responsible for survive of the Mugil cephalus against Aeromonas hydrophila infection. The MALDI TOF sample 08 and 09 were recognized as hypothetical proteins based on the MALDI TOF sample. A cytidinedeaminase was found in samples 10, 11, and 12. Extracellular proteins may be involved for the immunological increase in Mugil cephalus against Aeromonas hydrophila, according to the current research.
从鲻鱼中分离了 27 个细菌菌落的文化微生物组，以分析其抗菌和拮抗活性。使用 16S rRNA 测序对一种有效的益生菌进行了表征。将有效菌株添加到鱼饲料中进行挑战试验，以研究生长和免疫参数。收集并使用 MALDI-TOF/TOF 对益生菌的胞外蛋白进行了表征。在 G27 中，G9 菌株抑制了所有 5 种致病性菌株。分离出的细菌被鉴定为枯草芽孢杆菌 PRBD09，其登录号为 KF765648。经过 35 天的喂养期，枯草芽孢杆菌 PRBD09 增强了细胞和体液免疫反应，这有助于鲻鱼抵抗嗜水气单胞菌感染的存活。基于 MALDI-TOF 样本，MALDI-TOF 样本 08 和 09 被识别为假设蛋白。在样本 10、11 和 12 中发现了胞苷脱氨酶。根据目前的研究，胞外蛋白可能参与了鲻鱼对嗜水气单胞菌的免疫增强。
