Hassan Marwa A, Fathallah Mustafa A, Elzoghby Mohamed A, Salem Mohamed G, Helmy Mohamed S
Department of Animal Hygiene, Zoonoses and Behavior, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Fish Farming and Technology Institute, Suez Canal University, Ismailia, 41522, Egypt.
AMB Express. 2022 Feb 26;12(1):22. doi: 10.1186/s13568-022-01370-5.
The effects of two probiotics on NH degradation, as well as the magnetic field (21.56 m tesla) on the germination and proliferation of Bacillus spores, were studied in-vitro. Additionally, the effect of these probiotics on water quality maintenance in Litopenaeus vannamei holding ponds was investigated. For 180 min, NH degradation was assessed as follows: Set 1: ammonia-free tap water with NH; Probiotic A (5 × 10 viable Bacillus spores/g) with NH; Probiotic B (multi spp. 2 × 10 CFU/g) with NH; and Set 2: same as set 1 with 30 mg L OM. The magnetic field was tested on Probiotic A (3.5 × 10 CFU) for 36 h in triplicate. In the presence of organic matter, both probiotics degrade NH. The viable Bacillus count increased within 6 h of being exposed to the magnetic field, reaching its peak after 36 h. Firstly, fifteen ponds (250,000 PL/acre) were investigated, then 360 water samples were collected from the same corresponding pond for 8 weeks, and subjected to T1: control; T2: Probiotic A (0.007 g/m/2 weeks); T3: Probiotic B (0.03 g/m/2 weeks). Both probiotics with TVC and NH demonstrated a negative correlation, on the other hand, they showed a significant (P ≤ 0.01) improvement in DO and pH. Overall, both probiotics were able to degrade NH and the magnetic field (21.56 m tesla) was efficient to improve the germination and proliferation of Bacillus spores in-vitro. Probiotics were also effective for reducing TVC and NH levels by increasing dissolved oxygen and pH in pond water.
体外研究了两种益生菌对氨氮（NH）降解的影响，以及磁场（21.56毫特斯拉）对芽孢杆菌孢子萌发和增殖的影响。此外，还研究了这些益生菌对凡纳滨对虾养殖池塘水质维持的影响。在180分钟内，氨氮降解评估如下：第1组：含氨氮的无氨自来水；含氨氮的益生菌A（5×10个活芽孢杆菌孢子/克）；含氨氮的益生菌B（多种菌2×10 CFU/克）；第2组：与第1组相同，但添加30毫克/升的有机物质（OM）。对益生菌A（3.5×10 CFU）进行了36小时的磁场测试，重复三次。在有有机物质存在的情况下，两种益生菌都能降解氨氮。暴露于磁场6小时内，活芽孢杆菌数量增加，36小时后达到峰值。首先，对15个池塘（每英亩250,000尾虾苗）进行了调查，然后在8周内从同一相应池塘采集了360个水样，并分为T1：对照组；T2：益生菌A（0.007克/立方米/2周）；T3：益生菌B（0.03克/立方米/2周）。两种益生菌的总活菌数（TVC）与氨氮均呈负相关，另一方面，它们在溶解氧（DO）和pH值方面有显著（P≤0.01）改善。总体而言，两种益生菌都能降解氨氮，磁场（21.56毫特斯拉）在体外能有效促进芽孢杆菌孢子的萌发和增殖。益生菌还通过增加池塘水中的溶解氧和pH值，有效降低了总活菌数和氨氮水平。
