Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
Internal Medicine Research Unit, Pfizer, Cambridge, MA, USA.
Methods Mol Biol. 2022;2440:211-222. doi: 10.1007/978-1-0716-2051-9_13.
Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains of zebrafish larvae and intact embryos, and discuss the considerations involved in the imaging of nonflat, whole-organ or organism samples, more broadly.
自 2015 年问世以来，扩展显微镜技术（ExM）使在多种模型中以纳米级分辨率对各种生物结构进行成像成为可能。在这里，我们详细描述了在斑马鱼幼虫和完整胚胎的整个大脑中应用 ExM 的方案，并更广泛地讨论了对非平面、整个器官或生物体样本进行成像所涉及的注意事项。
