使用传统抗体和荧光蛋白的扩展显微镜技术。
Expansion microscopy with conventional antibodies and fluorescent proteins.
作者信息
Chozinski Tyler J, Halpern Aaron R, Okawa Haruhisa, Kim Hyeon-Jin, Tremel Grant J, Wong Rachel O L, Vaughan Joshua C
机构信息
Department of Chemistry, University of Washington, Seattle, Washington, USA.
Department of Biological Structure, University of Washington, Seattle, Washington, USA.
出版信息
Nat Methods. 2016 Jun;13(6):485-8. doi: 10.1038/nmeth.3833. Epub 2016 Apr 11.
Abstract
Expansion microscopy is a technique in which fluorophores on fixed specimens are linked to a swellable polymer that is physically expanded to enable super-resolution microscopy with ordinary microscopes. We have developed and characterized new methods for linking fluorophores to the polymer that now enable expansion microscopy with conventional fluorescently labeled antibodies and fluorescent proteins. Our methods simplify the procedure and expand the palette of compatible labels, allowing rapid dissemination of the technique.
摘要
扩展显微镜技术是一种将固定标本上的荧光团与可膨胀聚合物相连的技术，该聚合物经物理膨胀后，可使用普通显微镜进行超分辨率显微镜观察。我们已开发并表征了将荧光团与聚合物相连的新方法，现在使用传统荧光标记抗体和荧光蛋白即可实现扩展显微镜技术。我们的方法简化了操作流程，扩大了兼容标记物的范围，使该技术能够迅速推广。
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