Todorović-Raković Nataša, Milovanović Jelena, Greenman John, Radulovic Marko
Department of Experimental Oncology, Institute of Oncology and Radiology of Serbia, Belgrade, Serbia.
Department of Biomedical Sciences, University of Hull, Hull, UK.
Cytokine. 2022 Apr;152:155836. doi: 10.1016/j.cyto.2022.155836. Epub 2022 Feb 23.
Interferon-γ (IFN-γ) is a pleiotropic immunomodulatory cytokine. Because of its contradictory and even dualistic roles in malignancies, its potential as a biomarker remains to be unraveled.
To evaluate the prognostic significance of serum IFN-γ in hormonally treated breast cancer patients.
The study included 72 premenopausal breast cancer patients with known clinicopathological characteristics. All patients received adjuvant hormonal therapy based on hormone receptor-positivity. The median follow-up period was 93 months. IFN-γ serum protein levels were determined by quantitative ELISA. Prognostic performance was evaluated by the receiver operating characteristic (ROC), Cox proportional hazards regression and Kaplan-Meier analyses. Classification of patients into IFN-γ and IFN-γ subgroups was performed by the use of the outcome-oriented cut-off point categorization approach.
The best prognostic performance was achieved by IFN-γ (AUC = 0.24 and p = 0.01 for distant events, AUC = 0.29 and p = 0.01 for local and distant events combined). Age and IFN-γ were prognostically significant in instances of all types of outcomes and IFN-γ was the independent prognostic parameter (Cox regression). There was a significant difference between IFN-γ values of patients without any events and those with distant metastases (Mann-Whitney test, p = 0.007). IFN-γ levels correlated significantly with nodal status and tumor stage (Spearman's rank order, r = -0.283 and r = -0.238, respectively). Distant recurrence incidence was 4% for the IFN-γ subgroup and 33% for the IFN-γ subgroup (Kaplan-Meier analysis).
Raised serum IFN-γ levels associate independently with favorable disease outcome in hormonally dependent breast cancer.
干扰素-γ（IFN-γ）是一种具有多效性的免疫调节细胞因子。由于其在恶性肿瘤中作用相互矛盾甚至呈二元性，其作为生物标志物的潜力仍有待揭示。
评估血清IFN-γ在接受激素治疗的乳腺癌患者中的预后意义。
本研究纳入72例具有已知临床病理特征的绝经前乳腺癌患者。所有患者均基于激素受体阳性接受辅助激素治疗。中位随访期为93个月。采用定量ELISA法测定血清IFN-γ蛋白水平。通过受试者工作特征（ROC）曲线、Cox比例风险回归和Kaplan-Meier分析评估预后性能。采用以结果为导向的切点分类方法将患者分为IFN-γ高和IFN-γ低亚组。
IFN-γ显示出最佳的预后性能（远处事件的AUC = 0.24，p = 0.01；局部和远处事件合并的AUC = 0.29，p = 0.01）。在所有类型的预后情况中，年龄和IFN-γ具有预后意义，且IFN-γ是独立的预后参数（Cox回归）。无任何事件的患者与有远处转移的患者的IFN-γ值之间存在显著差异（Mann-Whitney检验，p = 0.007）。IFN-γ水平与淋巴结状态和肿瘤分期显著相关（Spearman等级相关，r分别为-0.283和-0.238）。IFN-γ低亚组的远处复发发生率为4%，IFN-γ高亚组为33%（Kaplan-Meier分析）。
血清IFN-γ水平升高与激素依赖性乳腺癌的良好疾病预后独立相关。
