Department of Gastroenterology, Haimen Hospital Affiliated to Nantong University, Nantong 226100, Jiangsu, China.
Department of Gastroenterology, Affiliated Hospital of Jiangnan University, Wuxi 214122, Jiangsu, China.
Pathol Res Pract. 2022 Apr;232:153744. doi: 10.1016/j.prp.2021.153744. Epub 2021 Dec 17.
Colorectal carcinoma (CRC) represents a most grave healthy burden worldwide. TESTIN has been confirmed as a predictive biomarker for several cancers. In the present study, we sought to assess the expression level and prognostic values of TESTIN in CRC.
The levels of TESTIN mRNA and protein were detected in 132 paired CRC tissues and noncancerous ones via quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) assays, respectively. Chi-square test was adopted to analyze the association of TESTIN expression with clinicopathological profiles of CRC patients. To explore prognostic value of TESTIN, Kaplan-Meier curve and Cox regression analyses were employed.
TESTIN expression was down-regulated among CRC tissues in comparison to bordering cancer-free samples at both protein and mRNA levels (P < 0.001). Decreased TESTIN expression was closely related to poor tumor differentiation (P = 0.001) and advanced TNM stages (P = 0.001). CRC cases with low expression of TESTIN were more likely to undergo dismal overall survivals (log-rank P = 0.003). Multivariate Cox analysis unveiled that down-regulated expression of TESTIN was independently correlated with poor prognosis (HR=2.422, 95% CI=1.294-4.535, P = 0.006).
The down-regulation of TESTIN may predict dismal prognosis for CRC patients.
结直肠癌（CRC）是全球最严重的健康负担之一。TESTIN 已被证实是几种癌症的预测性生物标志物。在本研究中，我们旨在评估 TESTIN 在 CRC 中的表达水平和预后价值。
通过定量实时聚合酶链反应（qRT-PCR）和免疫组织化学（IHC）检测 132 对 CRC 组织和非癌组织中 TESTIN mRNA 和蛋白的水平。采用卡方检验分析 TESTIN 表达与 CRC 患者临床病理特征的关系。采用 Kaplan-Meier 曲线和 Cox 回归分析探讨 TESTIN 的预后价值。
与癌旁无肿瘤组织相比，CRC 组织中 TESTIN 的蛋白和 mRNA 表达均下调（P<0.001）。TESTIN 表达下调与肿瘤分化不良（P=0.001）和 TNM 分期较晚（P=0.001）密切相关。TESTIN 低表达的 CRC 患者总生存率较差（log-rank P=0.003）。多因素 Cox 分析显示，TESTIN 表达下调与预后不良独立相关（HR=2.422，95%CI=1.294-4.535，P=0.006）。
TESTIN 的下调可能预示 CRC 患者预后不良。
