Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India; Academy of Scientific & Innovative Research (AcSIR), CSIR- Human Resource Development Centre (CSIR-HRDC), Ghaziabad, Uttar Pradesh, 201 002, India.
Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176 061, India.
J Environ Manage. 2022 May 15;310:114764. doi: 10.1016/j.jenvman.2022.114764. Epub 2022 Feb 23.
Microbial remediation of synthetic dyes from industrial effluents offers a sustainable and eco-friendly alternative. Herein, laccase-producing bacteria were isolated from decaying wood niches in the Himalayan region. A bacterial consortium (BC-I) was developed to decolorize synthetic dyes cocktail of three major groups (azo, anthraquinone, and triphenylmethane). BC-I consisted of Klebsiella sp. PCH427, Enterobacter sp. PCH428, and Pseudomonas sp. PCH429 can decolorize 77% of 240 mg/L dyes cocktail in 44 h at 37 °C. BC-I works under wide pH (4.0-10.0), a high salt concentration (NaCl, 10%), and low nutrients. Further, FT-IR and LC-MS validated the dyes cocktail degradation and identified the degraded products. Additionally, phytotoxicity analysis of BC-I treated dyes cocktail significantly reduced the toxicity to Vigna radiata and Cicer arietinum compared to untreated dyes cocktail. The present study has simulated environmental challenges of acidic, alkaline, and saline industrial dyes effluents, which are significant to bioremediation.
从工业废水中去除合成染料的微生物修复提供了一种可持续的环保替代方法。本研究从喜马拉雅山地区腐烂的木材缝隙中分离出产漆酶的细菌。开发了一种细菌联合体 (BC-I) 来对三种主要类型（偶氮、蒽醌和三苯甲烷）的合成染料混合物进行脱色。BC-I 由 Klebsiella sp. PCH427、Enterobacter sp. PCH428 和 Pseudomonas sp. PCH429 组成，可在 37°C 下 44 小时内将 240mg/L 染料混合物的 77%脱色。BC-I 在宽 pH 值（4.0-10.0）、高盐浓度（NaCl，10%）和低养分条件下均能发挥作用。此外，FT-IR 和 LC-MS 验证了染料混合物的降解，并鉴定了降解产物。此外，与未经处理的染料混合物相比，BC-I 处理后的染料混合物的植物毒性分析显著降低了对 Vigna radiata 和 Cicer arietinum 的毒性。本研究模拟了酸性、碱性和含盐工业染料废水的环境挑战，这对生物修复具有重要意义。
