Institute of Chemical Industry of Forest Products, CAF, National Engineering Laboratory for Biomass Chemical Utilization, Key and Open Laboratory on Forest Chemical Engineering, SFA, Key Laboratory of Biomass Energy and Material, Nanjing 210042, Jiangsu Province, China; College of Materials Science and Technology, Beijing Forestry University, 100083 Beijing, China; Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, NKUA, 15771 Athens, Greece.
Division of Pharmaceutical Technology, Department of Pharmacy, NKUA, 15771 Athens, Greece.
Food Chem. 2022 Aug 1;384:132470. doi: 10.1016/j.foodchem.2022.132470. Epub 2022 Feb 16.
Oleocanthal, oleacein, oleuropein and hydroxytyrosol comprise characteristic polyphenols of olive with high biological value. However, stability problems hinder their further investigation. Thus, in the present study they were incorporated in nanoliposomes by thin film hydration method. The particles sizes, PDI, zeta-potential and physicochemical stabilities of nanoliposomes were evaluated by light scattering methods while FTIR, XRD, TGA and DSC methods were carried out for further physicochemical characterization. Their micromorphology was illustrated by negative-staining TEM and Cryo-TEM, revealing well-dispersed round-shaped vesicles. According to in vitro release studies, oleocanthal and oleacein were rapidly released in a higher percentage than oleuropein and hydroxytyrosol and compatible with the Ritger-Peppas model release mechanism while only oleuropein liposomes were governed by anomalous diffusion of non-Fickian diffusion. Antioxidant assays showed that nanoliposomes presented comparable activity with pure compounds enabling them as suitable carriers for the delivery of olive active biophenols in the human organism.
油橄榄多酚（包括油橄榄苦苷、橄榄苦苷、羟基酪醇和橄榄多酚）是具有高生物价值的橄榄油的特征性多酚。然而，其稳定性问题阻碍了对它们的进一步研究。因此，本研究采用薄膜水化法将它们包封于纳米脂质体中。通过光散射法评估了纳米脂质体的粒径、PDI、Zeta 电位和物理化学稳定性，而通过傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、热重分析（TGA）和差示扫描量热法（DSC）进行了进一步的物理化学特性研究。通过负染 TEM 和冷冻 TEM 观察到它们的微观形态，呈现出良好分散的圆形囊泡。根据体外释放研究，油橄榄多酚和油橄榄苦苷的释放速度较快，释放比例高于橄榄多酚和羟基酪醇，符合 Ritger-Peppas 模型释放机制，而只有橄榄多酚脂质体遵循非菲克扩散的异常扩散机制。抗氧化试验表明，纳米脂质体与纯化合物具有相当的活性，使其成为在人体中输送橄榄活性生物酚的合适载体。
