Derayea Sayed M, Madian Hoda, Samir Ebtihal, Hamad Ahmed A, Badr El-Din Khaled M
Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia 61519, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia 61519, Egypt; Analytical Chemistry Department, Deraya University, New Minia, Minia 61519, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 May 15;273:121024. doi: 10.1016/j.saa.2022.121024. Epub 2022 Feb 10.
In the present work a new, feasible, and green approach was employed for the analysis of milnacipran. A drug is used in the management of depression in addition to fibromyalgia. It inhibits of the reuptake of two essential neurotransmitters serotonin and nor-adrenaline. In slightly alkaline buffer (pH 8.5) the primary amino group of milnacipran reacted with fluorescamine to give a substituted pyrrolone derivative which exhibited high fluorescence activity. The fluorescence of produced derivative was measured at (λ 385 nm, λ 477 nm), and the experimental factors were cautiously optimized. The measured intensity of fluorescence was plotted versus the respective concentration of milnacipran to setup the calibration plot which has a linear concentration range of 50-300 ng/mL. The ICH guidelines were utilized to totally validate the presented approach. In addition the method could be efficiently incorporated in the analysis of commercial milnacipram tablets with no considerable effect on the results of the assay for milnacipran. The developed approach is characterized by its high simplicity and greenness of the procedure which make it suitable for routine analysis.
在本研究中，采用了一种新的、可行的绿色方法来分析米那普明。除用于治疗纤维肌痛外，该药物还用于治疗抑郁症。它可抑制两种重要神经递质血清素和去甲肾上腺素的再摄取。在微碱性缓冲液（pH 8.5）中，米那普明的伯氨基与荧光胺反应，生成具有高荧光活性的取代吡咯烷酮衍生物。在（λ 385 nm，λ 477 nm）处测量生成衍生物的荧光，并谨慎优化实验因素。将测得的荧光强度与米那普明的相应浓度作图，建立校准曲线，其线性浓度范围为50 - 300 ng/mL。采用国际协调会议（ICH）指南对所提出的方法进行全面验证。此外，该方法可有效地用于市售米那普明片剂的分析，对米那普明的含量测定结果没有显著影响。所开发的方法具有高度的简便性和绿色性，适用于常规分析。
