Hamad Ahmed Abdulhafez
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assiut Branch Assiut 71524 Egypt.
RSC Adv. 2023 Mar 21;13(14):9361-9369. doi: 10.1039/d2ra08183k. eCollection 2023 Mar 20.
l-Tetramisole is an anti-nematode and immunomodulating agent employed medically to treat diseases caused by worms. It is also used as an immune system-modifying agent in rheumatoid arthritis and as assistant therapy in treating cancers in the colorectal, head, and neck regions. Felumin is a safe food dye used in feed additives, flavors, biostaining, and recently, in analytical tracking. This is the first innovative spectrofluorimetric approach to l-Tetramisole drug analysis. It is both efficient and environmentally benign and was evaluated and validated in this investigation using a green, one-pot, and direct spectrofluorimetric technique. Instant complexes were created by combining l-Tetramisole and Felumin in an acidic solution. The fluorometric investigation was performed based on the off-effect strategy of l-Tetramisole on the emission amplitude of a biological dye (Felumin) at 557.5 nm. The linear range was from 0.1 to 1.7 μg mL, with sensitivity limits of 0.020 and 0.061 μg mL, respectively. Analytical modulation of l-Tetramisole-Felumin complexes was done for all system parameters. The system was found to meet ICH criteria. Moreover, this technique successfully recovered the substance in the prescribed medicinal dosage forms. The created fluorimetric technique was also successfully employed to track the drug of interest in human biofluids, which was a major accomplishment. The kinetics of the reaction system was also studied further. Finally, the proposed method's environmental friendliness was evaluated on the eco-scale.
左旋四咪唑是一种抗线虫和免疫调节药物，用于医学上治疗由蠕虫引起的疾病。它还被用作类风湿性关节炎的免疫系统调节剂以及结直肠癌、头颈癌治疗的辅助疗法。荧光素是一种安全的食用色素，用于饲料添加剂、香料、生物染色，最近还用于分析追踪。这是第一种用于左旋四咪唑药物分析的创新型荧光光谱法。它既高效又环保，在本研究中使用绿色、一锅法直接荧光光谱技术进行了评估和验证。在酸性溶液中，将左旋四咪唑和荧光素混合形成即时络合物。荧光测定研究基于左旋四咪唑对生物染料（荧光素）在557.5nm处发射幅度的猝灭效应策略。线性范围为0.1至1.7μg/mL，检测限分别为0.020和0.061μg/mL。对左旋四咪唑 - 荧光素络合物的所有系统参数进行了分析调节。该系统符合国际协调会议（ICH）标准。此外，该技术成功回收了规定剂型中的药物。所创建的荧光光谱技术还成功用于追踪人体生物流体中的目标药物，这是一项重大成就。还进一步研究了反应体系的动力学。最后，在生态尺度上评估了所提出方法的环境友好性。
