Endocrinology and Nephrology Unit, CHU de Québec-Laval University Research Center, Quebec, Québec, Canada.
Department of Kinesiology, Laval University, Quebec, Québec, Canada.
Lifestyle Genom. 2022;15(3):77-86. doi: 10.1159/000523809. Epub 2022 Feb 25.
MicroRNA (miRNA) profiles have been shown to change after intake of dairy products. Dysregulation of miRNA is associated with the changes in the level of glycemic parameters. The objectives are: (1) to investigate miRNA expression after consumption of dairy products and (2) to study the association between miRNAs and glycemic profile among individuals with hyperinsulinemia.
In crossover design, 24 participants were randomized into 2 phases: high dairy (HD) (≥4 servings/day according to the Canadian food guide [2007]) and adequate dairy (AD) (≤2 servings/day) over 6 weeks. First, miRNAs were extracted from a pooled plasma sample of 10 subjects after HD and AD intervention which analyzed in duplicate by array hybridization (Affymetrix Gene Chip miRNA Array v. 4.0). Second, 6 miRNAs related to type 2 diabetes (T2D) were validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) from plasma of 24 participants.
Microarray analysis indicated that 297 miRNAs expressed differentially (FC ≥ ±1.2; p value <0.05) in a pooled plasma sample of 10 subjects. Among pooled miRNAs, the level of selected miRNAs, including miR-652-3p, miR-106b-5p, miR-93-5p, and miR-107 were downregulated; conversely, miR-223-3p and miR-122-5p were upregulated. After qRT-PCR validation, only the expression level of miR-106-5p tended to be increased after HD compared to AD (p = 0.06). After AD intervention, the level of fasting plasma glucose (FPG) and insulin and homeostatic model assessment of insulin resistance were negatively correlated with miR-122-5p. Similarly, negative correlation was found between miR-106-5p and FPG.
The miRNAs profile was modified after HD compared to AD, and this may have role in modifying the risk of T2D (registration No. NCT02961179).
已经证明，摄入乳制品后 miRNA 谱会发生变化。miRNA 的失调与血糖参数水平的变化有关。目的是：（1）研究食用乳制品后 miRNA 的表达情况，（2）研究高胰岛素血症个体中 miRNA 与血糖谱之间的关系。
采用交叉设计，将 24 名参与者随机分为 2 期：高乳制品（HD）（根据加拿大食品指南[2007 年]≥4 份/天）和适量乳制品（AD）（≤2 份/天），持续 6 周。首先，在 HD 和 AD 干预后，从 10 名受试者的混合血浆样本中提取 miRNA，并用阵列杂交（Affymetrix Gene Chip miRNA Array v. 4.0）进行重复分析。其次，从 24 名参与者的血浆中验证了与 2 型糖尿病（T2D）相关的 6 个 miRNA 通过定量逆转录聚合酶链反应（qRT-PCR）。
微阵列分析表明，在 10 名受试者的混合血浆样本中，有 297 个 miRNA 表达差异（FC ≥ ±1.2；p 值<0.05）。在混合 miRNA 中，包括 miR-652-3p、miR-106b-5p、miR-93-5p 和 miR-107 的水平下调；相反，miR-223-3p 和 miR-122-5p 上调。经过 qRT-PCR 验证，与 AD 相比，HD 后 miR-106-5p 的表达水平仅呈升高趋势（p=0.06）。AD 干预后，空腹血糖（FPG）和胰岛素水平以及胰岛素稳态模型评估的胰岛素抵抗与 miR-122-5p 呈负相关。同样，miR-106-5p 与 FPG 之间也存在负相关。
与 AD 相比，HD 后 miRNA 谱发生了改变，这可能在改变 T2D 的风险中起作用（注册号 NCT02961179）。
