高胰岛素血症患者食用高乳制品后基因表达谱的调节

Modulation of gene expression profile following consumption of high-dairy products in subjects with hyperinsulinemia.

作者信息

Khorraminezhad Leila, Rudkowska Iwona

机构信息

Endocrinology and Nephrology Unit, CHU de Québec-Laval University Research Center, Quebec, Canada.

Endocrinology and Nephrology Unit, CHU de Québec-Laval University Research Center, Quebec, Canada; Department of Kinesiology, Laval University, Québec (QC), Canada.

出版信息

Nutr Metab Cardiovasc Dis. 2023 Jan;33(1):219-226. doi: 10.1016/j.numecd.2022.10.015. Epub 2022 Nov 1.

DOI:10.1016/j.numecd.2022.10.015

PMID:36411217

Abstract

BACKGROUND AND AIM

Dysregulation of gene expression is associated to a higher risk of type 2 diabetes (T2D). Further, research indicates that dairy consumption may potentially affect gene expression. The aim of this study was to examine if genes and pathways associated with T2D are differentially changed in subjects with hyperinsulinemia after high dairy (HD) diet.

METHODS AND RESULTS

Ten obese patients with hyperinsulinemia who consumed HD (4 servings/day according to the Canadian Food Guide (2007)) for six weeks participated in this study. Before and after HD consumption, fasting blood samples were collected. Blood was taken in PAX-gene tubes and RNA was extracted and analyzed using Clariom S microarrays. Results indicated that 236 genes (137 up-regulated and 99 down-regulated; fold change (FC) ≥ ±1.2; p < 0.05) were expressed differentially between before and after HD intake. Genes related to pathways associated with insulin signaling and inflammation, such as olfactory receptor activity, G-protein-coupled receptors (GPCR), phosphatidylinositol-3-OHKinase (PI3K)/AKT2 (PI3K-AKT2), Ras signaling, Mitogen-Activated Protein Kinase (MAPK) were altered following HD.

CONCLUSION

Overall, results suggest a potential protective effect of HD intake in individuals at risk of T2D through modification of gene expression profiles.

REGISTRATION NUMBER FOR CLINICAL STUDIES

NCT02961179.

摘要

背景与目的

基因表达失调与2型糖尿病（T2D）的高风险相关。此外，研究表明食用乳制品可能会潜在影响基因表达。本研究的目的是检验在高乳制品（HD）饮食后，高胰岛素血症患者中与T2D相关的基因和通路是否发生了差异变化。

方法与结果

10名患有高胰岛素血症的肥胖患者参与了本研究，他们按照加拿大食物指南（2007年）的标准食用HD（每天4份），为期六周。在食用HD之前和之后，采集空腹血样。血液采集于PAXgene管中，提取RNA并使用Clariom S微阵列进行分析。结果表明，在摄入HD前后，有236个基因（137个上调和99个下调；倍数变化（FC）≥±1.2；p<0.05）表达存在差异。与胰岛素信号传导和炎症相关通路的基因，如嗅觉受体活性、G蛋白偶联受体（GPCR）、磷脂酰肌醇-3-OH激酶（PI3K）/AKT2（PI3K-AKT2）、Ras信号传导、丝裂原活化蛋白激酶（MAPK）在HD后发生了改变。