Endocrinology and Nephrology Unit, CHU de Québec-Laval University Research Center, CHUL-2705, Quebec City, QC G1V 4G2, Canada.
Department of Medicine, Laval University, Quebec City, QC G1V 4G2, Canada.
Nutrients. 2021 Jun 18;13(6):2088. doi: 10.3390/nu13062088.
F-IsoProstanes (F-IsoPs) are major biomarkers of oxidative stress and are associated with type 2 diabetes (T2D). Further, plasma levels of F-IsoPs may be modified by dairy products. The aim is to investigate the effect of high dairy product (HD) consumption compared to an adequate dairy product (AD) consumption on the level of F-IsoPs among hyperinsulinemic subjects. In this crossover study, participants were randomized in two groups: HD (≥4 servings/day), or AD (≤2 servings/day) for six weeks. Fasting blood glucose and insulin were measured. The homeostatic model assessment of insulin resistance (HOMA-IR) was calculated. Six isomers of F-IsoPs were quantified by HPLC-MS/MS. Twenty-seven subjects with hyperinsulinemia (mean age; 55 ± 13 years, BMI; 31.4 ± 3.3 kg/m2) were included. Fasting glucose, insulin and HOMA-IR were unchanged after HD or AD intervention. After HD intake, the total level of F-IsoPs ( = 0.03), 5-F-IsoP ( = 0.002), and 8-F-IsoP ( = 0.004) decreased compared to AD. The 15-F-IsoP tended to be positively correlated with fasting blood glucose ( = 0.39, = 0.08). Generally, F-IsoPs levels were higher among men compared to women regardless of the dairy intake. Overall, intake of HD decreased plasma levels of F-IsoPs compared to AD without modifying glycemic parameters.
F-同型前列腺素（F-IsoPs）是氧化应激的主要生物标志物，与 2 型糖尿病（T2D）有关。此外，乳制品可能会改变 F-IsoPs 的血浆水平。本研究旨在调查与适量乳制品（AD）摄入相比，高乳制品（HD）摄入对高胰岛素血症患者 F-IsoPs 水平的影响。在这项交叉研究中，参与者被随机分为两组：HD（≥4 份/天）或 AD（≤2 份/天），持续 6 周。测量空腹血糖和胰岛素。计算稳态模型评估的胰岛素抵抗（HOMA-IR）。通过 HPLC-MS/MS 定量了 6 种 F-IsoPs 异构体。共纳入 27 例高胰岛素血症患者（平均年龄 55 ± 13 岁，BMI 31.4 ± 3.3 kg/m2）。HD 或 AD 干预后空腹血糖、胰岛素和 HOMA-IR 均无变化。与 AD 相比，HD 摄入后总 F-IsoPs 水平（ = 0.03）、5-F-IsoP（ = 0.002）和 8-F-IsoP（ = 0.004）降低。15-F-IsoP 与空腹血糖呈正相关趋势（ = 0.39， = 0.08）。无论乳制品摄入量如何，F-IsoPs 水平在男性中均高于女性。总体而言，与 AD 相比，HD 摄入降低了 F-IsoPs 的血浆水平，而血糖参数没有改变。
