Cüce Mustafa, Sezgin Muslu Asiye
Department of Food Technology, Şebinkarahisar School of Applied Sciences, Giresun University, Giresun, Turkey.
Department of Biology, Faculty of Science, Karadeniz Technical University, 61080 Trabzon, Turkey.
Physiol Mol Biol Plants. 2022 Jan;28(1):289-299. doi: 10.1007/s12298-022-01132-4. Epub 2022 Jan 31.
Paraquat (PQ) causes oxidative stress, the main source of damage in plants subjected to adverse environmental factors. Sodium nitroprusside (SNP), a signaling molecule, alleviates oxidative damage. The present study was carried out to investigate the role of exogenous SNP in the amelioration of PQ-mediated oxidative stress effects on plantlets cultured in MS basal media. Firstly, MS medium supplemented with 6-BA was found to be the best basal medium for seed germination. Then, a rapid micropropagation protocol was designed to produce . plantlets by using nodal segments as explants, and 0.25 mg/L 6-BA in combination with 0.1 mg/L IBA was found to be the most favorable for shoot proliferation of . . Four weeks old plants were applied with or without SNP (100 μM) and exposed to oxidative stress induced by 2.5 μM PQ. The SNP application decreased membrane damage, hydrogen peroxide, and proline contents, and increased relative water, pigments, ascorbate and total phenolic contents, and some antioxidant enzyme activities in seedlings under PQ stress compared to PQ stress alone. These results suggested that exogenous SNP could protect plantlets propagated in vitro with PQ stress through modulation of proline and phenolics biosynthesis and antioxidant defense system.
百草枯（PQ）会引发氧化应激，这是遭受不利环境因素影响的植物受损的主要来源。硝普钠（SNP）作为一种信号分子，可减轻氧化损伤。本研究旨在探究外源SNP在改善PQ介导的对在MS基础培养基中培养的小植株的氧化应激效应方面所起的作用。首先，发现添加了6 - 苄基腺嘌呤（6 - BA）的MS培养基是种子萌发的最佳基础培养基。然后，设计了一种快速微繁殖方案，以节段为外植体来培育小植株，并且发现0.25毫克/升的6 - BA与0.1毫克/升的吲哚丁酸（IBA）组合对[具体植物名称]的芽增殖最为有利。对四周龄的植株施加或不施加SNP（100微摩尔），并使其暴露于由2.5微摩尔PQ诱导的氧化应激环境中。与仅施加PQ应激相比，施加SNP降低了膜损伤、过氧化氢和脯氨酸含量，增加了相对含水量、色素、抗坏血酸和总酚含量以及PQ胁迫下幼苗中的一些抗氧化酶活性。这些结果表明，外源SNP可通过调节脯氨酸和酚类物质的生物合成以及抗氧化防御系统，保护在PQ胁迫下离体繁殖的小植株。
