Kim Tae Hyung, Kim Ji Su, Kwon Ji Eun, Park Bumhee, Lee Eun-So
Department of Dermatology, Ajou University School of Medicine, Suwon, Korea.
Office of Biostatistics, Medical Research Collaborating Center, Ajou Research Institute for Innovative Medicine, Ajou University Medical Center, Suwon, Korea.
Ann Dermatol. 2022 Feb;34(1):7-13. doi: 10.5021/ad.2022.34.1.7. Epub 2022 Jan 27.
Palmoplantar pustulosis (PPP) is initiated from the acrosyringium. However, it is unclear whether PPP should be considered a distinct entity or should be classified into the spectrum of pustular psoriasis, also known as palmoplantar pustular psoriasis (PPPP).
We evaluated the differences in immunohistochemical staining in patients with PPP to determine whether they can be classified into two groups based on psoriatic properties or acrosyringeal properties.
Nineteen punch biopsy specimens diagnosed with PPP were collected. Antibodies were chosen for identifying the acrosyringeal properties of α-3-nicotine acetylcholine receptors (α-3-nAChR), psoriatic properties of interleukin (IL)-23 and IL-36R, inflammatory cell properties of human cathelicidin antimicrobial peptide 18/LL-37, IL-8, lipocalin-2 (LCN2), and CD3. The degree of staining of the epidermis was evaluated using the ordinal scale (0~3). The principal component analysis was used to derive principal components (PCs) of common variation between the stains, and the two groups were divided using PCs and cluster analysis.
Three main PCs explained 64% of the total variance in PPP. PC1 (pustular psoriasis properties) showed a higher correlation with IL-36R. PC2 (acrosyringeal/inflammatory properties) showed a higher correlation with α-3-nAChR, IL-8, LCN2, and CD3. PC3 (psoriasis properties) showed a higher correlation with IL-23. PC1 showed a statistically significant difference (=0.0284) between the two groups. We identified three PCs associated with the pathomechanisms of PPP.
Although PC1 showed a statistically significant difference between the two groups, we did not identify differential protein expression related to the pathogenesis between PPP and PPPP.
掌跖脓疱病（PPP）起源于汗腺导管末端。然而，尚不清楚PPP应被视为一种独立的疾病实体，还是应归类于脓疱型银屑病谱，即掌跖脓疱型银屑病（PPPP）。
我们评估了PPP患者免疫组化染色的差异，以确定是否可根据银屑病特性或汗腺导管末端特性将患者分为两组。
收集19例诊断为PPP的打孔活检标本。选择抗体来识别α-3-烟碱乙酰胆碱受体（α-3-nAChR）的汗腺导管末端特性、白细胞介素（IL）-23和IL-36受体的银屑病特性、人抗菌肽18/LL-37、IL-8、脂质运载蛋白-2（LCN2）和CD3的炎症细胞特性。使用序数量表（0~3）评估表皮的染色程度。主成分分析用于得出染色之间共同变异的主成分（PC），并使用PC和聚类分析将两组分开。
三个主要PC解释了PPP总方差的64%。PC1（脓疱型银屑病特性）与IL-36R的相关性更高。PC2（汗腺导管末端/炎症特性）与α-3-nAChR、IL-8、LCN2和CD3的相关性更高。PC3（银屑病特性）与IL-23的相关性更高。两组之间PC1有统计学显著差异（=0.0284）。我们确定了与PPP发病机制相关的三个PC。
虽然两组之间PC1有统计学显著差异，但我们未发现PPP和PPPP之间与发病机制相关的差异蛋白表达。
