Peng Li, Wen Lijuan, Zhang Jie, Zhang Xiaotong, Wei Qin, Guo Jing, Zeng Jinhao
Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Front Pharmacol. 2022 Feb 9;12:639580. doi: 10.3389/fphar.2021.639580. eCollection 2021.
Paeoniflorin (PF) is a monoterpene glucoside with various biological properties, and it suppresses allergic and inflammatory responses in a rat model of urticaria-like lesions (UL). In the present study, we treated OVA-induced mice presenting UL with PF at four circadian time points (ZT22, ZT04, ZT10, and ZT16) to determine the optimal administration time of PF. The pharmacological effects of PF were assessed by analyzing the scratching behavior; histopathological features; allergic responses such as immunoglobulin E (IgE), leukotriene B4 (LTB4), and histamine (HIS) release; inflammatory cell infiltration [mast cell tryptase (MCT) and eosinophil protein X (EPX)]; and mRNA levels of inflammatory cytokines such as interleukin (IL)-12, IL-6, interferon-γ (IFN-γ), and IL-4. It was demonstrated that PF significantly alleviated scratching behavior and histopathological features, and ZT10 dosing was the most effective time point in remission of the condition among the four circadian time points. Moreover, PF decreased the serum levels of IgE, LTB4, and HIS, and PF administration at ZT10 produced relatively superior effectiveness. PF treatment, especially dosing at ZT10, significantly reduced the number of mast cells and granules and diminished the infiltration of MCT and EPX in the skin tissues of mice with UL. Furthermore, the oral administration of PF effectively decreased the inflammatory cytokine levels of IL-12 mRNA. In conclusion, different administration times of PF affected its efficacy in mice with UL. ZT10 administration demonstrated relatively superior effectiveness, and it might be the optimal administration time for the treatment of urticaria.
芍药苷（PF）是一种具有多种生物学特性的单萜糖苷，它可抑制类荨麻疹样病变（UL）大鼠模型中的过敏和炎症反应。在本研究中，我们在四个昼夜时间点（ZT22、ZT04、ZT10和ZT16）用PF处理呈现UL的卵清蛋白（OVA）诱导小鼠，以确定PF的最佳给药时间。通过分析抓挠行为、组织病理学特征、过敏反应（如免疫球蛋白E（IgE）、白三烯B4（LTB4）和组胺（HIS）释放）、炎症细胞浸润（肥大细胞类胰蛋白酶（MCT）和嗜酸性粒细胞蛋白X（EPX））以及炎症细胞因子（如白细胞介素（IL）-12、IL-6、干扰素-γ（IFN-γ）和IL-4）的mRNA水平来评估PF的药理作用。结果表明，PF显著减轻了抓挠行为和组织病理学特征，并且在四个昼夜时间点中，ZT10给药是病情缓解最有效的时间点。此外，PF降低了血清中IgE、LTB4和HIS的水平，在ZT10给药产生的效果相对更优。PF治疗，尤其是在ZT10给药，显著减少了肥大细胞和颗粒的数量，并减少了UL小鼠皮肤组织中MCT和EPX的浸润。此外，口服PF有效降低了IL-12 mRNA的炎症细胞因子水平。总之，PF的不同给药时间影响其对UL小鼠的疗效。ZT10给药显示出相对更优的效果，可能是治疗荨麻疹的最佳给药时间。
