Jia Dongsheng, Luo Guozhong, Shi Wei, Liu Ye, Liu Huan, Zhang Xiaofeng, Wei Taiyun
Vector-borne Virus Research Center, Fujian Province Key Laboratory of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, China.
Front Microbiol. 2022 Feb 11;13:834712. doi: 10.3389/fmicb.2022.834712. eCollection 2022.
Rice stripe mosaic virus (RSMV), a newly discovered plant cytorhabdovirus, and rice gall dwarf virus (RGDV), a plant reovirus, are transmitted by leafhopper in a persistent-propagative manner. In this study, field surveys in Luoding city, Guangdong province of southern China, showed that RSMV and RGDV frequently co-infected rice plants. Furthermore, this co-infection had a synergistic effect on viral replication potential and pathogenicity in rice plants. Meanwhile, RSMV and RGDV also co-infected vectors, and RGDV significantly promoted the propagation of RSMV in co-infected vectors. Accordingly, co-infection significantly promoted the acquisition and transmission efficiencies of RSMV by . However, such co-infection did not significantly affect the propagation of RGDV in vectors. More importantly, we also observed that non-viruliferous preferred to feed on co-infected rice plants, and this process further affected the feeding behavior of to enhance viral release into rice phloem. These results provided the clues as to why RSMV had been a gradually expanding problem, creating an increasing risk of damage to rice production. Our findings revealed that synergism between RSMV and RGDV in their host and vector enhanced the propagation and transmission of RSMV, which will help guide the formulation of viral control strategies.
水稻条纹花叶病毒（RSMV）是一种新发现的植物细胞质弹状病毒，而水稻瘤矮病毒（RGDV）是一种植物呼肠孤病毒，它们均由叶蝉以持久性增殖方式传播。在本研究中，对中国南方广东省罗定市进行的田间调查显示，RSMV和RGDV经常共同侵染水稻植株。此外，这种共同侵染对水稻植株中的病毒复制潜力和致病性具有协同作用。同时，RSMV和RGDV也共同侵染介体，并且RGDV显著促进了RSMV在共同侵染介体中的增殖。因此，共同侵染显著提高了叶蝉对RSMV的获毒和传毒效率。然而，这种共同侵染并未显著影响RGDV在介体中的增殖。更重要的是，我们还观察到无毒叶蝉更喜欢取食共同侵染的水稻植株，而这一过程进一步影响了叶蝉的取食行为，从而增强了病毒向水稻韧皮部的释放。这些结果为RSMV为何逐渐成为一个不断扩大的问题、给水稻生产造成越来越大的损害风险提供了线索。我们的研究结果表明，RSMV和RGDV在其寄主和介体中的协同作用增强了RSMV的增殖和传播，这将有助于指导制定病毒防治策略。
