Du Bingyao, Meng Lu, Liu Huimin, Zheng Nan, Zhang Yangdong, Zhao Shengguo, Wang Jiaqi
State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2022 Feb 10;13:853263. doi: 10.3389/fmicb.2022.853263. eCollection 2022.
In this study, we investigated the species composition and diversity of psychrotrophic bacteria in raw milk from Heilongjiang, Inner Mongolia, Gansu, Henan, Anhui, Jiangsu, Chongqing, and Hunan provinces in China using traditional cultivation and PacBio Single Molecule Real-Time sequencing methods. The isolated psychrotrophic bacteria were highly diverse, which composed of 21 genera and 59 species. accounted for 58.9% of the total genera while and were also highly represented (above 5.0%). In particular, occurred at a level of 16.9% and were present in relatively high proportions (above 4.0%). Regional differences were found significantly among the test regions except samples from Heilongjiang and Inner Mongolia were similar. Additionally, differences were observed between days in Henan, Anhui, and Jiangsu samples. Therefore, control strategies must be implemented on regional and season basis.
在本研究中，我们运用传统培养法和PacBio单分子实时测序方法，对中国黑龙江、内蒙古、甘肃、河南、安徽、江苏、重庆和湖南等省份生鲜乳中嗜冷菌的种类组成和多样性进行了调查。分离出的嗜冷菌具有高度多样性，由21个属和59个种组成。 占总属数的58.9%，而 和 也占比较高（超过5.0%）。特别是， 占比达16.9%， 存在比例相对较高（超过4.0%）。除黑龙江和内蒙古的样本相似外，测试区域之间存在显著的区域差异。此外，在河南、安徽和江苏的样本中，不同日期之间也存在差异。因此，必须根据区域和季节实施控制策略。
