Hu Xiaohong, Liu Lingying, Wang Yu, Yu Yonghui, Li Zhongyuan, Liu Yanan, Chai Jiake
Department of Pediatrics in the Fourth Medical Center, Chinese PLA General Hospital, Beijing 100048, China.
Department of Nutrition in the Fourth Medical Center, Chinese PLA General Hospital, Beijing 100048, China.
Stem Cells Int. 2022 Feb 18;2022:8661689. doi: 10.1155/2022/8661689. eCollection 2022.
To investigate whether hUC-MSCs attenuated severe burn-induced ALI and the effects were based on TSG-6 secreted from hUC-MSCs.
A rat model was established and evaluated as follows: cytokine expression was measured by ELISA, and both inflammatory cell infiltration and lung injury were assessed by immunohistochemistry assay.
In vitro, TSG-6 levels in serum from the burn group were significantly increased compared with those from the sham group. In vivo, TSG-6 levels of lung tissues and serum in the burn+hUC-MSC group were significantly increased compared with those in the burn group. Both in lung tissues and in serum, increased levels of proinflammatory cytokines (TNF-, IL-1, and IL-6) were remarkably decreased, but the anti-inflammatory cytokine IL-10 increased after hUC-MSC administration ( < 0.05). These significant positive effects after hUC-MSC transplantation did not occur in the burn+siTSG-6 group.
The intratracheal implantation of hUC-MSCs has been an effective treatment for severe burn-induced ALI via promoting TSG-6 secretion and inhibiting inflammatory reaction in lung tissue.
研究人脐带间充质干细胞（hUC-MSCs）是否能减轻严重烧伤诱导的急性肺损伤（ALI），以及其作用是否基于hUC-MSCs分泌的肿瘤坏死因子诱导蛋白6（TSG-6）。
建立大鼠模型并进行如下评估：通过酶联免疫吸附测定（ELISA）测量细胞因子表达，通过免疫组织化学分析评估炎症细胞浸润和肺损伤。
在体外，烧伤组血清中的TSG-6水平与假手术组相比显著升高。在体内，烧伤+hUC-MSCs组肺组织和血清中的TSG-6水平与烧伤组相比显著升高。在肺组织和血清中，促炎细胞因子（肿瘤坏死因子-α、白细胞介素-1和白细胞介素-6）水平显著降低，但给予hUC-MSCs后抗炎细胞因子白细胞介素-10升高（P<0.05）。在烧伤+siTSG-6组中未出现hUC-MSCs移植后的这些显著积极作用。
经气管植入hUC-MSCs通过促进TSG-6分泌和抑制肺组织炎症反应，已成为治疗严重烧伤诱导的ALI的有效方法。
